Nicholson G A, Hawkins S, McLeod J G
Department of Medicine, University of Sydney, Australia.
Muscle Nerve. 1990 Aug;13(8):675-80. doi: 10.1002/mus.880130803.
A number of phosphoproteins were found in the soluble fraction of rat skeletal muscle by two-dimensional polyacrylamide gel electrophoresis (PAGE). The pattern of some of these phosphoproteins differed in fast (extensor digitorum longus, EDL) or slow (soleus) muscles, was dependent on normal innervation, and was altered with denervation. In order to determine if the pattern was maintained by electrical activity or trophic factors, we compared the effect of electrical block by local neural application of tetrodotoxin with the effect of complete nerve section. Both methods produced similar alterations in phosphoproteins, indicating that the pattern is dependent on nerve activity, not trophic factors. Such phosphoproteins are possible mediators of neural activity on gene expression.
通过二维聚丙烯酰胺凝胶电泳（PAGE）在大鼠骨骼肌的可溶性部分发现了许多磷酸化蛋白。其中一些磷酸化蛋白的模式在快肌（趾长伸肌，EDL）或慢肌（比目鱼肌）中有所不同，依赖于正常的神经支配，并且会随着去神经支配而改变。为了确定这种模式是由电活动还是营养因子维持的，我们比较了局部神经应用河豚毒素进行电阻断的效果与完全切断神经的效果。两种方法都导致磷酸化蛋白发生了类似的变化，表明这种模式依赖于神经活动，而非营养因子。这类磷酸化蛋白可能是神经活动对基因表达的介导物。
