细胞质HuR表达：与口腔鳞状细胞癌细胞中凋亡蛋白2细胞抑制剂表达及临床病理因素的相关性

Cytoplasmic HuR expression: correlation with cellular inhibitors of apoptosis protein-2 expression and clinicopathologic factors in oral squamous cell carcinoma cells.

作者信息

Cha Jeong-Dan, Kim Hee-Kyeong, Cha In-Ho

机构信息

Department of Research Development, Institute of Jinan Red Ginseng, Jinan-gun, South Korea.

出版信息

Head Neck. 2014 Aug;36(8):1168-75. doi: 10.1002/hed.23431. Epub 2014 Jan 16.

DOI:10.1002/hed.23431

PMID:23852810

Abstract

BACKGROUND

HuR expression has been noted in several cancer types, in which it may contribute to increased expression of cellular inhibitors of apoptosis protein-2 (cIAP2) observed during tumorigenesis.

METHODS

To assess the correlation between cIAP2 and HuR in cases of oral squamous cell carcinoma (OSCC), the expression patterns of HuR and cIAP2 were assessed by immunohistochemical analyses of 95 treated OSCC samples.

RESULTS

In the tumor tissues, positive cytoplasmic HuR expression was evident in 71.6% of samples and positive cIAP2 expression was noted in 95.8% of samples. Positive cytoplasmic HuR expression was significantly associated with positive cIAP2 (p < .035) and high cIAP2 expression (p < .007), as well as high grade (p < .050). The inhibition of HuR expression by small interfering RNA or leptomycin B caused a reduction in the inducibility of cIAP2 in oral cancer cells.

CONCLUSION

Cytoplasmic expression of HuR is associated with cIAP2 expression in OSCCs.

摘要

背景

HuR在多种癌症类型中均有表达，在肿瘤发生过程中，它可能促使凋亡抑制蛋白2（cIAP2）的表达增加。

方法

为评估口腔鳞状细胞癌（OSCC）病例中cIAP2与HuR之间的相关性，通过对95例接受治疗的OSCC样本进行免疫组化分析，评估HuR和cIAP2的表达模式。

结果

在肿瘤组织中，71.6%的样本中可见HuR在细胞质呈阳性表达，95.8%的样本中cIAP2呈阳性表达。细胞质HuR阳性表达与cIAP2阳性（p < 0.035）、cIAP2高表达（p < 0.007）以及高级别（p < 0.050）显著相关。小干扰RNA或 leptomycin B抑制HuR表达会导致口腔癌细胞中cIAP2的诱导性降低。