Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
Chemistry. 2013 Aug 19;19(34):11148-52. doi: 10.1002/chem.201301832. Epub 2013 Jul 12.
Enzymatic amino acid synthesis: Kinetic resolution and asymmetric synthesis of various valuable 3-substituted aspartic acids, which were obtained in fair to good yields with diastereomeric ratio values of up to >98:2 and enantiomeric excess values of up to >99 %, by using engineered methylaspartate ammonia lyases are described. These biocatalytic methodologies for the selective preparation of aspartic acid derivatives appear to be attractive alternatives for existing chemical methods.
通过使用工程化的天冬氨酸氨甲酰基转移酶，对各种有价值的 3-取代天冬氨酸进行动力学拆分和不对称合成，以获得高达>98:2 的非对映体比率值和高达>99%的对映体过量值，这些方法获得了良好至优秀的产率。这些用于选择性制备天冬氨酸衍生物的生物催化方法似乎是现有化学方法的有吸引力的替代方法。
