Department of Chemistry, University of South Florida, 4202 E. Fowler Ave. CHE 205, Tampa, FL 33620, USA.
Chembiochem. 2013 Aug 19;14(12):1434-7. doi: 10.1002/cbic.201300264. Epub 2013 Jul 12.
Which came first? Using thermodynamic cycles constructed from experimental measures of the binding free energy and free energy simulations, we have shown that binding of Sulfolobus acidocaldarius protein Sac7d to DNA occurs before DNA bending, thus indicating that a conformational selection mechanism is unlikely to be operative in Sac7d binding.
我们利用从结合自由能的实验测量和自由能模拟中构建的热力学循环表明，嗜热嗜酸古菌蛋白 Sac7d 与 DNA 的结合发生在 DNA 弯曲之前，因此表明构象选择机制不太可能在 Sac7d 结合中起作用。
