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采用反相高效液相色谱法测定氧化槐果碱的pKa值。

pKa determination of oxysophocarpine by reversed - phase high performance liquid chromatography.

作者信息

Huo Huiling, Li Tingting, Zhang Lei

机构信息

College of Chinese Traditional Medicine, Guangzhou University of Traditional Chinese Medicine, 232 East of Waihuan Road, University Town, Guangzhou, Guangdong 510006 R.P. China ; College of Chinese Traditional Medicine, Southern Medical University, Guangzhou, Guangdong 510515 R.P. China.

出版信息

Springerplus. 2013 Jun 20;2(1):270. doi: 10.1186/2193-1801-2-270. Print 2013 Dec.

DOI:10.1186/2193-1801-2-270
PMID:23853749
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3701144/
Abstract

BACKGROUND

In this study, a RP - HPLC method was applied for determination of pKa value by using the dependence of the capacity factor (k) on the pH of the mobile phase for oxysophocarpine (OSP).

FINDINGS

The effect of the mobile phase composition on the ionization constant was studied by measuring the pKa value at different MeOH concentrations, ranging from 10 to 20% (v/v). Based on all pH - k curves plotted and pH values at inflection point calculated, experimental pKa value obtained for oxysophocarpine was 6.5.

CONCLUSION

This method was successfully applied to realize low sample consumption, rapid sample throughput, high sensitivity and precision.

摘要

背景

在本研究中，采用反相高效液相色谱法（RP - HPLC），利用氧化槐果碱（OSP）的容量因子（k）对流动相pH值的依赖性来测定其pKa值。

研究结果

通过在10%至20%（v/v）的不同甲醇浓度下测量pKa值，研究了流动相组成对电离常数的影响。根据绘制的所有pH - k曲线以及计算出的拐点处的pH值，氧化槐果碱的实验pKa值为6.5。

结论

该方法成功应用于实现低样品消耗、快速样品通量、高灵敏度和精密度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c5/3701144/3910df7e46e5/40064_2013_352_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c5/3701144/da87de893c2d/40064_2013_352_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c5/3701144/3910df7e46e5/40064_2013_352_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c5/3701144/da87de893c2d/40064_2013_352_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c5/3701144/3910df7e46e5/40064_2013_352_Fig2_HTML.jpg

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