Department of Environmental Chemistry & Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Torun, Poland.
Analyst. 2013 Sep 21;138(18):5221-9. doi: 10.1039/c3an00371j. Epub 2013 Jul 15.
The chromatographic properties of new N,O-dialkyl phosphoramidate stationary phases were studied. Obtained stationary phases containing C10 (Amino-P-C10) and C18 alkyl chains (Amino-P-C18) were attached to aminopropyl silica using the phosphate group. As basic characteristics of the bonded phases, the hydrophobicity and silanol activity (polarity) were investigated. Columns were compared according to the methylene selectivities using a series of alkylbenzene homologues and according to their shape and size selectivities using polycyclic aromatic hydrocarbons (PAHs). The measurements were done using methanol (MeOH)-water and acetonitrile (ACN)-water mobile phases. The presented results show that the coverage density of the bonded ligands and the length of the alkyl chains strongly influence the retention and selectivity of the N,O-dialkyl phosphoramidate bonded phases in reversed-phase liquid chromatography. The presence of the polar amino and phosphoramidate groups in the structure of the bonded ligands strongly influence the polarity of the bonded phase. A higher hydrophobicity is exhibited by the Amino-P-C18 adsorbent. The chromatographic properties of the new stationary phases were compared with octadecyl, cholesterol-bonded and immobilized artificial membrane (IAM) stationary phases.
研究了新型 N,O-二烷基磷酰胺固定相的色谱性能。通过使用磷酸基团，将含有 C10（氨基-P-C10）和 C18 烷基链（氨基-P-C18）的获得的固定相连接到氨丙基硅上。作为键合相的基本特性，研究了疏水性和硅醇活性（极性）。通过使用一系列烷基苯同系物，根据亚甲基选择性对色谱柱进行了比较，并通过使用多环芳烃（PAHs）根据其形状和大小选择性对色谱柱进行了比较。测量是使用甲醇（MeOH）-水和乙腈（ACN）-水流动相进行的。所呈现的结果表明，键合配体的覆盖密度和烷基链的长度强烈影响反相液相色谱中 N,O-二烷基磷酰胺键合相的保留和选择性。键合配体结构中存在极性氨基和磷酰胺基团强烈影响键合相的极性。氨基-P-C18 吸附剂表现出更高的疏水性。新型固定相的色谱性能与十八烷基、胆固醇键合和固定化人工膜（IAM）固定相进行了比较。
