Discipline of Anatomy & Histology, Bosch Institute, University of Sydney, Sydney 2006, Australia.
Heart Lung Circ. 2013 Oct;22(10):819-26. doi: 10.1016/j.hlc.2013.06.006. Epub 2013 Jul 13.
This Methodological Review is intended as a guide for research students who may have just discovered a human "novel" cardiac protein, but it may also help hard-pressed reviewers of journal submissions on a "novel" protein reported in an animal model of human heart failure. Whether you are an expert or not, you may know little or nothing about this particular protein of interest. In this review we provide a strategic guide on how to proceed. We ask: How do you discover what has been published (even in an abstract or research report) about this protein? Everyone knows how to undertake literature searches using PubMed and Medline but these are usually encyclopaedic, often producing long lists of papers, most of which are either irrelevant or only vaguely relevant to your query. Relatively few will be aware of more advanced search engines such as Google Scholar and even fewer will know about Quertle. Next, we provide a strategy for discovering if your "novel" protein is expressed in the normal, healthy human heart, and if it is, we show you how to investigate its subcellular location. This can usually be achieved by visiting the website "Human Protein Atlas" without doing a single experiment. Finally, we provide a pathway to discovering if your protein of interest changes its expression level with heart failure/disease or with ageing.
这篇方法学综述旨在为可能刚刚发现人类“新型”心脏蛋白的研究学生提供指导，但它也可能有助于苦苦挣扎的期刊投稿评审人员了解在心力衰竭的人类动物模型中报道的“新型”蛋白。无论您是否是专家，您可能对这个特别感兴趣的蛋白知之甚少或一无所知。在这篇综述中，我们提供了一个策略性的指导，告诉您如何进行。我们会问：您如何发现有关该蛋白的已发表内容（即使是摘要或研究报告）？每个人都知道如何使用 PubMed 和 Medline 进行文献检索，但这些通常是百科全书式的，经常会产生大量的论文，其中大多数与您的查询无关或只有模糊的相关性。相对较少的人会知道更高级的搜索引擎，如 Google Scholar，甚至更少的人会知道 Quertle。接下来，我们提供了一种策略来发现您的“新型”蛋白是否在正常、健康的人心肌中表达，如果是这样，我们将向您展示如何研究其亚细胞定位。这通常可以通过访问“人类蛋白质图谱”网站来实现，而无需进行任何实验。最后，我们提供了一条途径，以发现您感兴趣的蛋白的表达水平是否随着心力衰竭/疾病或衰老而变化。
