一株新分离的甲基杆菌（Methylobacterium rhodesianum H13）对二氯甲烷的有效利用。

Effective utilization of dichloromethane by a newly isolated strain Methylobacterium rhodesianum H13.

机构信息

College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China.

出版信息

Environ Sci Pollut Res Int. 2014 Jan;21(2):1010-9. doi: 10.1007/s11356-013-1972-7. Epub 2013 Jul 16.

DOI:10.1007/s11356-013-1972-7

Abstract

An effective dichloromethane (DCM) utilizer Methylobacterium rhodesianum H13 was isolated from activated sludge. A response surface methodology was conducted, and the optimal conditions were found to be 4.5 g/L Na2HPO4·12H2O, 0.5 g/L (NH4)2SO4, an initial pH of 7.55, and a temperature of 33.7 °C. The specific growth rate of 0.25 h(-1) on 10 mM DCM was achieved, demonstrating that M. rhodesianum H13 was superior to the other microorganisms in previous investigations of DCM utilization. DCM mineralization paralleled the production of cells, CO2, and water-soluble metabolites, as well as the release of Cl(-), whereas the carbon distribution and Cl(-) yield varied with DCM concentrations. The facts that complete degradation only occurred with DCM concentrations below 15 mM and repetitive degradation of 5 mM DCM could proceed for only three cycles were ascribed to pH decrease (from 7.55 to 3.02) though a buffer system was employed.

摘要

一株高效利用二氯甲烷（DCM）的甲基杆菌（Methylobacterium rhodesianum H13）从活性污泥中分离得到。采用响应面法，得出最佳条件为：4.5 g/L 的 Na2HPO4·12H2O、0.5 g/L 的(NH4)2SO4、初始 pH 值为 7.55、温度为 33.7°C。在 10 mM DCM 上的比生长速率达到 0.25 h(-1)，表明 M. rhodesianum H13 在以前的 DCM 利用微生物研究中优于其他微生物。DCM 的矿化与细胞、CO2 和水溶性代谢产物的产生以及 Cl(-)的释放平行进行，而碳分布和 Cl(-)产率随 DCM 浓度的变化而变化。尽管采用了缓冲体系，但只有在 DCM 浓度低于 15 mM 时才会发生完全降解，而 5 mM DCM 的重复降解只能进行三个循环，这归因于 pH 值的下降（从 7.55 下降到 3.02）。