巴西南马托格罗索州利用 PCR 监测锥虫在三锥虫中的感染。
Monitoring Trypanosoma cruzi infection in triatomines using PCR in Mato Grosso do Sul, Brazil.
机构信息
Programa de Pós Graduação em Doenças Infecciosas e Parasitárias, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
出版信息
Rev Soc Bras Med Trop. 2013 May-Jun;46(3):277-80. doi: 10.1590/0037-8682-0024-2013.
INTRODUCTION
The aim of the present study was to assess the polymerase chain reaction (PCR) as a method for detecting Trypanosoma cruzi infection in triatomines that had been previously determined by microscopic examination in the State of Mato Grosso do Sul, Brazil.
METHODS
In total, 515 specimens were collected. Material from the digestive tract of each triatomine was analyzed for the presence of T. cruzi by microscopic examination and PCR using the 121/122 primer set.
RESULTS
Among the 515 specimens tested, 58 (11.3%) were positive by microscopy and 101 (19.61%) were positive by PCR and there was an association between the results of the techniques (χ2 = 53.354, p = 0.001). The main species of triatomine identified was T. sordida (95.5%)
CONCLUSIONS
The use of PCR in entomological surveillance may contribute to a better assessment of the occurrence of T. cruzi in triatomine populations.
简介
本研究旨在评估聚合酶链反应（PCR）作为一种检测巴西马托格罗索州先前通过显微镜检查确定为感染克氏锥虫的三锥虫的方法。
方法
共采集了 515 个标本。通过使用 121/122 引物对，对每只三锥虫的消化道材料进行显微镜检查和 PCR 分析，以检测 T. cruzi 的存在。
结果
在测试的 515 个标本中，58 个（11.3%）通过显微镜检查呈阳性，101 个（19.61%）通过 PCR 呈阳性，两种技术的结果之间存在关联（χ2 = 53.354，p = 0.001）。鉴定出的主要三锥虫物种是 T. sordida（95.5%）
结论
在昆虫学监测中使用 PCR 可能有助于更好地评估三锥虫种群中 T. cruzi 的发生情况。
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