Department of Chemistry, University of Warsaw, Warsaw, Poland.
Electrophoresis. 2013 Sep;34(18):2740-53. doi: 10.1002/elps.201300103. Epub 2013 Aug 19.
In this study, the chip gel electrophoresis with LIF detection was applied in protein profiling of fractionated and total extracts of maize standards. The sensitivity of such determinations can be enhanced by lyophilization of extracts or employing filtering and preconcentration with cutoff filters. Combinatorial peptide ligand library applied for sample processing prior to the electrophoretic analysis was, especially, an effective pretreatment step in the determination of low-abundance proteins. Several repeatable differences were observed for protein profiles between maize standards not containing the genetically modified organisms (GMOs) and those containing GMO, which can be potentially employed for identification of GMO in maize samples and foods of maize origin.
在这项研究中，应用芯片凝胶电泳与 LIF 检测技术对玉米标准品的分级和总提取物进行蛋白质谱分析。通过对提取物进行冻干或采用具有截止值的过滤器进行过滤和预浓缩，可以提高这些测定的灵敏度。在电泳分析之前，应用组合肽配体文库对样品进行处理，这是确定低丰度蛋白质的特别有效的预处理步骤。在不含转基因生物（GMO）的玉米标准品和含 GMO 的玉米标准品之间，观察到蛋白质谱存在几种可重复的差异，这可能用于鉴定玉米样品和玉米来源的食品中的 GMO。
