Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, China.
Asia Pac Psychiatry. 2013 Mar;5(1):11-6. doi: 10.1111/j.1758-5872.2012.00205.x. Epub 2012 Jul 18.
To explore the association between histone deacetylase-2 (HDAC2) and histone deacetylase-3 (HDAC3) gene polymorphisms and schizophrenia.
A total of 208 family trios consisting of fathers, mothers and affected offspring with schizophrenia were recruited as our subjects. Four tag SNPs on HDAC2 (rs10499080, rs6568819, rs2499618 and rs13204445) and two tag SNPs on HDAC3 (rs11741808, rs2530223) genes were selected. The Mass ARRAY Assay Design software (Sequenom) was used to design amplification and allele specific extension primers. The Hardy-Weinberg equilibrium (HWE) for genotypic distributions was tested using the chi-square goodness-of-fit test. Allelic association for a single tag SNP was analyzed by using family-based association tests including the haplotype-based haplotype relative risk (HHRR) test and the transmission disequilibrium test (TDT).
The genotypic distributions of HDAC2 SNPs rs6568819, rs2499618 and rs13204445 and HDAC3 SNPs rs11741808 and rs2530223 were all in Hardy-Weinberg equilibrium (P > 0.05). HHRR analysis revealed no associations between the SNPs and schizophrenia (P > 0.05). In addition, the TDT did not show any significant associations between HDAC2 and HDAC3 SNPs and schizophrenia (P > 0.05).
HDAC2 and HDAC3 might not be associated with schizophrenia in the Chinese population.
探讨组蛋白去乙酰化酶-2（HDAC2）和组蛋白去乙酰化酶-3（HDAC3）基因多态性与精神分裂症的关系。
共招募了 208 个包含父亲、母亲和患有精神分裂症的受影响后代的家族三胞胎作为研究对象。选择了 HDAC2 上的 4 个标记 SNP（rs10499080、rs6568819、rs2499618 和 rs13204445）和 HDAC3 上的 2 个标记 SNP（rs11741808、rs2530223）。使用 Mass ARRAY assay Design 软件（Sequenom）设计扩增和等位基因特异性延伸引物。使用卡方拟合优度检验测试基因型分布的 Hardy-Weinberg 平衡（HWE）。使用基于家族的关联测试，包括基于单体型的单体型相对风险（HHRR）检验和传递不平衡检验（TDT），分析单个标记 SNP 的等位基因关联。
HDAC2 SNPs rs6568819、rs2499618 和 rs13204445 以及 HDAC3 SNPs rs11741808 和 rs2530223 的基因型分布均符合 Hardy-Weinberg 平衡（P > 0.05）。HHRR 分析显示，SNP 与精神分裂症之间没有关联（P > 0.05）。此外，TDT 也没有显示出 HDAC2 和 HDAC3 SNP 与精神分裂症之间存在任何显著关联（P > 0.05）。
在中国人群中，HDAC2 和 HDAC3 可能与精神分裂症无关。
