Li Baiyuan, Xie Xiaolin, Zhang Xianjiao, Cai Zhuoping, Zhu Honghui
South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Wei Sheng Wu Xue Bao. 2013 Apr 4;53(4):379-89.
We isolated myxobacteria in saline-alkaline soils of Xinjiang using a prey-predator strategy where the prey bacteria can induce the predator myxobacteria to form visible fruiting body, and evaluated intrinsic relationships between prey and predator myxobacteria.
Sixteen bacteria with inductive effects of fruiting body formation were obtained, and then used as preys to isolate the myxobacteria.
A total of 55 myxobacteria strains were isolated from 25 soil samples, which were identified to the genera of Myxococcus, Corallococcus, Pyxidicoccus, Cystobacter, and Nannocystis. Besides, 6 unpurified isolates were believed to be myxobacteria. All the 16 prey bacteria had preferable inductive effects on Myxococcus spp., whereas Pyxidicoccus spp. and Cystobacter spp. were only induced by Gram-positive strains.
The prey-predator strategy provided a new and more effective way to isolate myxobacteria.
我们采用捕食者-猎物策略从新疆盐碱土中分离黏细菌，其中猎物细菌可诱导捕食性黏细菌形成可见的子实体，并评估猎物与捕食性黏细菌之间的内在关系。
获得了16株具有诱导子实体形成作用的细菌，然后将其用作猎物来分离黏细菌。
从25份土壤样品中共分离出55株黏细菌菌株，鉴定为黏球菌属、珊瑚球菌属、Pyxidicoccus属、孢囊杆菌属和侏囊菌属。此外，有6株未纯化的分离株被认为是黏细菌。所有16株猎物细菌对黏球菌属均有较好的诱导作用，而Pyxidicoccus属和孢囊杆菌属仅由革兰氏阳性菌株诱导。
捕食者-猎物策略为分离黏细菌提供了一种新的、更有效的方法。
