Yang Juan, Du Guocheng, Chen Jian, Fang Fang
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Wei Sheng Wu Xue Bao. 2013 Apr 4;53(4):403-8.
From oral cavity we isolated and characterized probiotic lactobacilli that could probability be applied to therapy and prevention of oral diseases.
Lactobacillus strains were isolated by plating the saliva and dental plaque of healthy donors on selective medium. Then the target strains were tested for inhibiting the growth of a Streptococcus mutans strain belonging to cariogenic pathogen species. Other properties such as production of extracellular polysaccharide and resistance to the antibacterial substances were also investigated.
Lactobacillus fermentum Y29, a strain with antimicrobial activity against Streptococcus mutans, was obtained from dental plaque. This strain was an extracellular polysaccharide producer, which corresponds to its aggregation ability. Moreover, L. fermentum Y29 showed resistance to 1.0 mg/mL lysozyme and 140 microg/g hydrogen peroxide that may guarantee its persistence in the complex oral niche.
Probiotic properties were characterized of an oral isolate L. fermentum Y29, which provided a possibility for its application in prevention and treatment of oral diseases.
从口腔中分离并鉴定可能应用于口腔疾病治疗和预防的益生菌乳酸菌。
通过将健康供体的唾液和牙菌斑接种在选择性培养基上分离乳酸菌菌株。然后测试目标菌株对属于致龋病原体物种的变形链球菌菌株生长的抑制作用。还研究了其他特性，如细胞外多糖的产生和对抗菌物质的抗性。
从牙菌斑中获得了对变形链球菌具有抗菌活性的发酵乳杆菌Y29菌株。该菌株是细胞外多糖产生菌，这与其聚集能力相对应。此外，发酵乳杆菌Y29对1.0mg/mL溶菌酶和140μg/g过氧化氢具有抗性，这可能保证其在复杂口腔环境中的持久性。
对口腔分离株发酵乳杆菌Y29的益生菌特性进行了表征，这为其在口腔疾病预防和治疗中的应用提供了可能性。
