[NOD1和NOD2在变应性鼻炎中的表达及糖皮质激素对其的调控]
[The expression of NOD1 and NOD2 and the regulation of glucocorticoids on them in allergic rhinitis].
作者信息
Hu Shuang, You Xuejun, Cao Pingping, Liu Zheng, Cui Yonghua
机构信息
Department of Otolaryngology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan,430030,China.
出版信息
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2013 Apr;27(8):393-6.
OBJECTIVE
To investigate the expression of NOD1 and NOD2 in the nasal mucosa of healthy individuals and allergic rhinitis(AR), and explored the regulation of glucocorticoids on them.
METHOD
RT-PCR and immunohistochemistry were used to detect the expression of NOD1 and NOD2 in nasal mucosa from healthy control and AR. Nasal explant culture was used to explore the effect of glucocorticoids on NOD1 and NOD2 expression.
RESULT
NOD1 and NOD2 mRNA expression level was significantly increased in AR compared with control. Immunohistochemical staining demonstrated that NOD1 and NOD2 were mainly expressed by epithelial cells and some unknown cells in lamina propria and there were significantly more positive staining cells were observed in AR tissue when compared with control. Glucocorticoids down-regulated NOD1 and NOD2 expression in AR.
CONCLUSION
NOD1 and NOD2 as two PRRs may take part in the pathogenesis of AR, glucocorticoids may play a therapeutical role on allergic rhinitis through down-regulated the expression of NOD1 and NOD2.
目的
研究健康个体和变应性鼻炎（AR）患者鼻黏膜中NOD1和NOD2的表达情况，并探讨糖皮质激素对其的调节作用。
方法
采用逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法检测健康对照者和AR患者鼻黏膜中NOD1和NOD2的表达。采用鼻外植体培养法探讨糖皮质激素对NOD1和NOD2表达的影响。
结果
与对照组相比，AR患者鼻黏膜中NOD1和NOD2 mRNA表达水平显著升高。免疫组织化学染色显示，NOD1和NOD2主要由上皮细胞以及固有层中一些未知细胞表达，与对照组相比，AR组织中观察到的阳性染色细胞明显更多。糖皮质激素可下调AR中NOD1和NOD2的表达。
结论
NOD1和NOD2作为两种模式识别受体可能参与AR的发病机制，糖皮质激素可能通过下调NOD1和NOD2的表达对变应性鼻炎发挥治疗作用。
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