Lü Mei, Dong Zhen, Yang Zhanquan
Department of Otorhinolaryngology-Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun 130031.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2002 Aug;16(8):423-5.
To study the relation between the level of IL-12 expression and the pathogenesis of allergic rhinitis, we investigated the expression of interleukin-12 (IL-12) mRNA in the nasal mucosa of experimental allergic rhinitis.
24 healthy guinea pigs were randomly divided into two groups. A model of allergic rhinitis (AR) in guinea pig was established by using ovalbumin intraperitoneal immunization and nasal antigen challenge. The nasal mucosa obtained from AR models as well as normal controls were studied for histopathological changes with stain HE, and expression of IL-12 mRNA with RT-PCR.
IL-12 was expressed in all the samples from AR models to normal controls. The mean expression level of IL-12 gene, however, in AR models was lower than that in normal controls (0.667 +/- 0.104 vs 0.847 +/- 0.071, P < 0.01).
IL-12 expression was decreased in the nasal mucosa of allergic rhinitis, which plays an important role in the pathogenesis of allergic rhinitis. It is also suggested that IL-12 replacement therapy may be a new approach to the treatment of allergic rhinitis.
为研究白细胞介素-12（IL-12）表达水平与变应性鼻炎发病机制之间的关系，我们对实验性变应性鼻炎鼻黏膜中IL-12 mRNA的表达进行了研究。
将24只健康豚鼠随机分为两组。采用卵清蛋白腹腔注射致敏及鼻腔抗原激发的方法建立豚鼠变应性鼻炎（AR）模型。对AR模型及正常对照的鼻黏膜进行苏木精-伊红（HE）染色观察组织病理学变化，采用逆转录-聚合酶链反应（RT-PCR）检测IL-12 mRNA的表达。
从AR模型到正常对照的所有样本中均有IL-12表达。然而，AR模型中IL-12基因的平均表达水平低于正常对照（0.667±0.104对0.847±0.071，P<0.01）。
变应性鼻炎鼻黏膜中IL-12表达降低，其在变应性鼻炎发病机制中起重要作用。提示IL-12替代疗法可能是治疗变应性鼻炎的一种新方法。
