Medical Faculty Ljubljana, Institute of Microbiology and Immunology, University of Ljubljana, Ljubljana, Slovenia.
Helicobacter. 2013 Dec;18(6):444-53. doi: 10.1111/hel.12068. Epub 2013 Jul 17.
The natural course of Helicobacter pylori infection, as well as the success of antibiotic eradication is determined by the immune response to bacteria. The aim of the study is to investigate how different Helicobacter pylori isolates influence the dendritic cells maturation and antigen-presenting function in order to elucidate the differences between Helicobacter pylori strains, isolated from the patients with successful antibiotic eradication therapy or repeated eradication failure.
Dendritic cells maturation and antigen presentation were monitored by flow cytometry analysis of the major histocompatibility complex class II (MHC-II), Toll-like receptor (TLR) and costimulatory molecules expression, and by determining cytokine secretion.
Dendritic cells stimulated with Helicobacter pylori isolated from patients with repeated antibiotic eradication failure expressed less human leukocyte antigen (HLA-DR), CD86, TLR-2, and interleukin-8 (IL-8) compared to Helicobacter pylori strains susceptible to antibiotic therapy; the latter expressed lower production of IL-10. Polymyxin B inhibition of lipopolysaccharide reduces IL-8 secretion in the group of Helicobacter pylori strains susceptible to antibiotic therapy. The differences in IL-8 secretion between both groups are lipopolysaccharide dependent, while the differences in secretion of IL-10 remain unchanged after lipopolysaccharide inhibition. Inhibitor of cathepsin X Mab 2F12 reduced the secretion of IL-6, and the secretion was significantly lower in the group of Helicobacter pylori strains isolated from patients with repeated antibiotic eradication failure.
Helicobacter pylori strains, susceptible/resistant to antibiotic eradication therapy, differ in their capability to induce DCs maturation and antigen-presenting function.
幽门螺杆菌感染的自然病程以及抗生素根除的成功与否取决于对细菌的免疫反应。本研究旨在研究不同的幽门螺杆菌分离株如何影响树突状细胞的成熟和抗原呈递功能，以阐明在抗生素根除治疗成功或反复根除失败的患者中分离出的幽门螺杆菌菌株之间的差异。
通过主要组织相容性复合体 II (MHC-II)、Toll 样受体 (TLR) 和共刺激分子表达的流式细胞术分析以及细胞因子分泌的测定来监测树突状细胞的成熟和抗原呈递。
与对抗生素治疗敏感的幽门螺杆菌菌株相比，来自反复抗生素根除失败患者的幽门螺杆菌分离株刺激的树突状细胞表达的人类白细胞抗原 (HLA-DR)、CD86、TLR-2 和白细胞介素-8 (IL-8) 较少；后者产生的白细胞介素-10 较少。多粘菌素 B 抑制脂多糖可减少对抗生素治疗敏感的幽门螺杆菌菌株组中 IL-8 的分泌。两组之间的 IL-8 分泌差异依赖于脂多糖，而脂多糖抑制后 IL-10 的分泌差异保持不变。组织蛋白酶 X 的抑制剂 Mab 2F12 减少了 IL-6 的分泌，且在来自反复抗生素根除失败患者的幽门螺杆菌分离株组中分泌明显更低。
对抗生素根除治疗敏感/耐药的幽门螺杆菌菌株在诱导树突状细胞成熟和抗原呈递功能方面存在差异。
