Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
Zoology Department, King Saud University, Riyadh 11451, Saudi Arabia.
Int J Syst Evol Microbiol. 2013 Sep;63(Pt 9):3515-3523. doi: 10.1099/ijs.0.053173-0. Epub 2013 Jul 16.
The morphology and infraciliature of two novel marine scuticociliates, Metanophrys orientalis spec. nov. and Uronemella sinensis spec. nov., collected from sandy beaches at Qingdao, China, were investigated using live observation and protargol-staining methods. Metanophrys orientalis spec. nov. is distinguished by the following characteristics: marine habitat and a slender to elongate oval body with pointed anterior end and rounded caudal end, in vivo about 25-50 µm long; buccal field about a quarter to a third of body length; nine or ten somatic kineties with dikinetids approximately in anterior half of body, monokinetids in posterior half; membranelles 1 and 2 almost equal in length and composed of two and three longitudinal rows of kinetids respectively; paroral membrane with zigzag structure extending anteriorly to middle portion of membranelle 2; contractile vacuole pore located at posterior end of somatic kinety 1. The genus Uronemella is redefined as follows: marine form with an elongate-elliptical or inverted pear-shaped body; apical plate conspicuous; buccal field about two-thirds of body length, cytostome subequatorially located; oral apparatus Uronema-like; somatic kineties comprising a mixture of dikinetids and monokinetids. Uronemella sinensis spec. nov. is recognized by having an elongate-elliptical body with truncated apical frontal plate, size in vivo about 25-35 × 15-20 µm, nine or ten somatic kineties, membranelle 1 consisting of two or three basal bodies, contractile vacuole pore at posterior end of somatic kinety 1. This study also compared the small-subunit rRNA gene sequences of these two species with other closely related species to show the sequence divergence, which ranged from 3.53 to 9.60%. Phylogenetic analyses support the contention that the genus Uronemella is monophyletic, while Metanophrys is non-monophyletic.
两种新型海洋旋毛科纤毛虫，东方拟鳞壳虫（Metanophrys orientalis spec. nov.）和中华旋口虫（Uronemella sinensis spec. nov.）的形态和纤毛结构，通过活体观察和蛋白银染色方法进行了研究。东方拟鳞壳虫（Metanophrys orientalis spec. nov.）的特征如下：海洋生境，细长至长椭圆形身体，前端尖，后端圆，活体约 25-50 µm 长；口围区约占体长的四分之一至三分之一；9 或 10 个体动片，双联体位于身体前半部分，单联体位于后半部分；口纤毛 1 和 2 几乎等长，分别由两列和三列联体组成；口膜有向前延伸到口纤毛 2 中部的锯齿状结构；伸缩泡孔位于体动片 1 的后端。旋口科的定义如下：海洋生境，长椭圆形或倒梨形身体；顶极板明显；口围区约占体长的三分之二，口位于赤道附近；口器类似于 Uronema；体动片由双联体和单联体混合组成。中华旋口虫（Uronemella sinensis spec. nov.）的特征是具有截顶的顶极板的长椭圆形身体，活体大小约为 25-35 µm×15-20 µm，9 或 10 个体动片，口纤毛 1 由两个或三个基体组成，伸缩泡孔位于体动片 1 的后端。本研究还比较了这两个物种与其他密切相关物种的小亚基 rRNA 基因序列，显示出序列差异为 3.53%至 9.60%。系统发育分析支持旋口科是单系的，而拟鳞壳虫是非单系的。
