Bernard M, Balny C, Banerjee R, Douzou P
Biochim Biophys Acta. 1975 Jun 26;393(2):389-95. doi: 10.1016/0005-2795(75)90067-7.
The kinetics of recombination of carbon monoxide to hemoglobin produced by total flash photolysis of its carboxyderivative are studied at low temperatures (down to --55 degrees C) in mixed hydroalcoholic solvents. The rates are found to be different in two solvents used, namely ethylene glycol/buffer and methanol/buffer; for the former, the rates at subzero temperatures are simply explained by cooling and are consistent with the activation energy as measured in aqueous solution, while those in methanol/buffer show evidence of a specific solvent effect. Values are reported for the rate constants and activation energies in the two solvents.
在混合水醇溶剂中，于低温（低至-55摄氏度）下研究了一氧化碳与通过其羧基衍生物的全闪光光解产生的血红蛋白的重组动力学。发现在所使用的两种溶剂中，即乙二醇/缓冲液和甲醇/缓冲液，速率有所不同；对于前者，零下温度下的速率可通过冷却简单解释，并且与在水溶液中测得的活化能一致，而在甲醇/缓冲液中的速率则显示出特定的溶剂效应。报告了两种溶剂中速率常数和活化能的值。
