Recombination of carbon monoxide with hemoglobin after flash photolysis of the carboxyderivative in mixed solvents at subzero temperature.

The kinetics of recombination of carbon monoxide to hemoglobin produced by total flash photolysis of its carboxyderivative are studied at low temperatures (down to --55 degrees C) in mixed hydroalcoholic solvents. The rates are found to be different in two solvents used, namely ethylene glycol/buffer and methanol/buffer; for the former, the rates at subzero temperatures are simply explained by cooling and are consistent with the activation energy as measured in aqueous solution, while those in methanol/buffer show evidence of a specific solvent effect. Values are reported for the rate constants and activation energies in the two solvents.