Bugrova V S, Ivanov R S, Bondar' I V
Ross Fiziol Zh Im I M Sechenova. 2013 Apr;99(4):453-63.
The impact of system anesthetic propofol on strength of functional response was investigated in population of primary visual cortex neurons. Elaborated protocol of anesthesia allowed appropriate depth of anesthesia and artifact free experimental data recording. At the start of experiment optical access to the surface of cortex was provided. Propofol was used as main general anesthetic. Control of anesthesia depth was carried out by monitoring of physiological parameters. Effect ofpropofol on functional response was evaluated by intrinsic optical imaging. Stability of neuronal response was estimated during whole experiment and after single injection ofpropofol. It was shown that extended administration ofpropofol do not have negative influence on strength of recorded neuronal signal. At the same time bolus of propofol depresses amplitude of response. Practical recommendations are suggested for conduction of longitudinal neurophysiological experiments using propofol as main anesthetic.
在初级视觉皮层神经元群体中研究了全身麻醉药丙泊酚对功能反应强度的影响。精心制定的麻醉方案可实现适当的麻醉深度并记录无伪迹的实验数据。实验开始时，提供了对皮质表面的光学观察通道。丙泊酚用作主要的全身麻醉药。通过监测生理参数来控制麻醉深度。通过内在光学成像评估丙泊酚对功能反应的影响。在整个实验过程中以及单次注射丙泊酚后评估神经元反应的稳定性。结果表明，长时间使用丙泊酚对记录的神经元信号强度没有负面影响。同时，丙泊酚推注会降低反应幅度。针对以丙泊酚为主要麻醉药进行纵向神经生理学实验提出了实用建议。
