Naughton Catherine, Corless Samuel, Gilbert Nick
MRC Human Genetics Unit; Institute of Genetics and Molecular Medicine; The University of Edinburgh; Edinburgh, UK.
Transcription. 2013 Jul-Aug;4(4):162-6. doi: 10.4161/trns.25554. Epub 2013 Jul 1.
New approaches using biotinylated-psoralen as a probe for investigating DNA structure have revealed new insights into the relationship between DNA supercoiling, transcription and chromatin compaction. We explore a hypothesis that divergent RNA transcription generates negative supercoiling at promoters facilitating initiation complex formation and subsequent promoter clearance.
使用生物素化补骨脂素作为探针来研究DNA结构的新方法，揭示了关于DNA超螺旋、转录和染色质压缩之间关系的新见解。我们探讨了一种假说，即不同方向的RNA转录在启动子处产生负超螺旋，促进起始复合物的形成以及随后的启动子清除。
