Borensztajn J, Kotlar T J
Department of Pathology, Northwestern University Medical School, Chicago, IL 60611.
Biochem J. 1990 Jul 15;269(2):539-42. doi: 10.1042/bj2690539.
The lipids extracted from chylomicrons, chylomicron remnants generated in vivo and hepatic-lipase-treated chylomicrons were emulsified by sonication. These emulsified particles retained the capacity of the native lipoproteins to be differentiated by the liver in vivo, i.e. only the particles derived from remnant and hepatic-lipase-treated chylomicron lipids were efficiently taken up by the liver. To investigate the role of phospholipids in this differentiation process, the phospholipids of all three lipoprotein preparations were separated from the remaining lipids by silicic acid chromatography. The phospholipid-free lipid fraction of chylomicrons was then emulsified with the phospholipids derived from each of the three lipoprotein preparations. Only the particles emulsified with phospholipids derived from remnants and hepatic-lipase-treated chylomicrons were efficiently taken up by the liver in vivo. These results support the proposition that phospholipids modulate the hepatic differentiation between chylomicrons and remnants in vivo.
从乳糜微粒、体内产生的乳糜微粒残粒以及经肝脂酶处理的乳糜微粒中提取的脂质，通过超声处理进行乳化。这些乳化颗粒保留了天然脂蛋白在体内被肝脏区分的能力，即只有源自残粒和经肝脂酶处理的乳糜微粒脂质的颗粒能被肝脏有效摄取。为了研究磷脂在这一区分过程中的作用，通过硅酸色谱法将所有三种脂蛋白制剂中的磷脂与其余脂质分离。然后，将乳糜微粒的无磷脂脂质部分与源自三种脂蛋白制剂中每一种的磷脂进行乳化。只有用源自残粒和经肝脂酶处理的乳糜微粒的磷脂乳化的颗粒在体内能被肝脏有效摄取。这些结果支持了磷脂在体内调节肝脏对乳糜微粒和残粒的区分这一观点。
