Zeng Junyan, Qu Qiumin
Department of Neurology, the First Affiliated Hospital of Medical College of Xi 'an Jiaotong University, Xi'an 710061, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2013 Jun;30(3):572-6.
The characteristics of virus-like particle (VLP) of JC virus (JCV) as a vector for targeting gene delivery was determined. The exogenous DNA (PUC19) packaged in VLP-Z was resistant to DNase I. VLP-Z was able to deliver a reporter plasmid pEGFP-N1 into HeLa cells and the green fluorescent reporter protein was expressed in these cells. VLP-Z was also able to bind IgG by interaction with the Z fragment of VLP-Z and IgG. These results suggested that VLP-Z might be used as a vector to deliver therapeutic genes to target cells with incorporating IgG antibodies.
确定了JC病毒（JCV）的病毒样颗粒（VLP）作为靶向基因递送载体的特性。包装在VLP-Z中的外源DNA（PUC19）对脱氧核糖核酸酶I具有抗性。VLP-Z能够将报告质粒pEGFP-N1递送至HeLa细胞，并且在这些细胞中表达绿色荧光报告蛋白。VLP-Z还能够通过与VLP-Z的Z片段和IgG相互作用结合IgG。这些结果表明，VLP-Z可能用作载体，通过掺入IgG抗体将治疗性基因递送至靶细胞。
