Akbas H, Dertlioglu S B, Dilmec F, Balkan M
Department of Medical Biology, University of Harran, Sanliurfa, Turkey.
Clin Ter. 2013 May-Jun;164(3):e173-7. doi: 10.7417/CT.2013.1564.
Vitiligo is an acquired hypomelanotic skin disorder resulting from the loss of functional melanocytes from the cutaneous epidermis. Low catalase (CAT) activity and Accumulation of hydrogen peroxide (H2O2) have been demonstrated in the epidermis of vitiligo patients. Some polymorphisms on catalase gene may have effect on the quantity and activity of catalase enzyme. The aim of this study was to investigate whether catalase (CAT) gene polymorphisms are associated with susceptibility to vitiligo in Turkish population.
One hundred and three patients with vitiligo and one hundred and seven gender, age and ethnic matched controls were enrolled in the study. Genotyping was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
The CAT gene -89A>T (rs7943316) and 389C>T (rs769217) genotypes and allele frequencies of vitiligo patients did not differ significantly from those of healthy controls.
We found no association between CAT gene -89A>T and 389C>T polymorphism and vitiligo susceptibility in Turkish vitiligo patients. Further studies with greater sample size should be performed to verify these results.
白癜风是一种后天性色素减退性皮肤病，由皮肤表皮功能性黑素细胞缺失所致。已证实在白癜风患者的表皮中过氧化氢酶（CAT）活性降低且过氧化氢（H2O2）蓄积。过氧化氢酶基因的一些多态性可能会对过氧化氢酶的数量和活性产生影响。本研究的目的是调查在土耳其人群中过氧化氢酶（CAT）基因多态性是否与白癜风易感性相关。
本研究纳入了103例白癜风患者以及107例性别、年龄和种族匹配的对照。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）进行基因分型。
白癜风患者的CAT基因-89A>T（rs7943316）和389C>T（rs769217）基因型及等位基因频率与健康对照无显著差异。
我们发现土耳其白癜风患者中CAT基因-89A>T和389C>T多态性与白癜风易感性之间无关联。应开展更大样本量的进一步研究以验证这些结果。
