Jones E C, McNeal J, Bruchovsky N, de Jong G
Department of Pathology, Vancouver General Hospital, British Columbia, Canada.
Cancer. 1990 Aug 15;66(4):752-7. doi: 10.1002/1097-0142(19900815)66:4<752::aid-cncr2820660426>3.0.co;2-1.
DNA content of prostatic adenocarcinoma was determined by flow cytometry on formalin-fixed, paraffin-embedded tissue from 57 radical prostatectomies. This was done to define the relationship of aneuploidy to prostatic adenocarcinoma grade, volume, and pathologic stage and to examine its utility in candidates for surgical treatment. Aneuploidy was found in 26 (46%) cases. With one exception, all of the aneuploid cases were found in tumors larger than 4 cc. The percentage of aneuploid cases increased with advancing pathologic stage, and it was highest in those cases with lymph node metastases. This percentage was also higher among more poorly differentiated tumors. However, diploid tumors were also found among these groups, and the relationship between aneuploidy versus pathologic stage and grade did not achieve statistical significance. Except for a 91% specificity for tumor volume greater than 4 cc, the sensitivity and specificity of DNA content analysis to predict these groups was low (50% to 72%). It is concluded that aneuploidy is a later event linked to tumor progression, but it is not a requirement for progression to occur. The overlap in aneuploid and diploid tumor behavior precludes the use of DNA content analysis as an independent predictor to direct preoperative treatment of prostatic carcinoma.
通过流式细胞术对57例根治性前列腺切除术的福尔马林固定、石蜡包埋组织进行前列腺腺癌的DNA含量测定。这样做是为了明确非整倍体与前列腺腺癌分级、体积及病理分期之间的关系，并检验其在手术治疗候选患者中的效用。在26例（46%）病例中发现了非整倍体。除1例例外，所有非整倍体病例均见于体积大于4立方厘米的肿瘤中。非整倍体病例的百分比随病理分期进展而增加，在有淋巴结转移的病例中最高。在分化较差的肿瘤中该百分比也更高。然而，在这些组中也发现了二倍体肿瘤，非整倍体与病理分期及分级之间的关系未达到统计学意义。除了对肿瘤体积大于4立方厘米有91%的特异性外，DNA含量分析预测这些组的敏感性和特异性较低（50%至72%）。结论是非整倍体是与肿瘤进展相关的后期事件，但它不是进展发生的必要条件。非整倍体和二倍体肿瘤行为的重叠使得无法将DNA含量分析用作指导前列腺癌术前治疗的独立预测指标。
