Vegeto E, Cocciolo M G, Raspagliesi F, Piffanelli A, Fontanelli R, Maggi A
Milano Molecular Pharmacology Laboratory, University of Milan, Italy.
Cancer Res. 1990 Sep 1;50(17):5291-5.
The levels of progesterone receptor (PR) mRNA, PR protein, and [3H]R5020 binding activity were measured in parallel experiments conducted on a T47D subline expressing the estrogen receptor. A significant increase of PR mRNA levels could be detected within 6 h of exposure of the cells to estradiol (10(-8) M). The changes in mRNA, however, did not lead to any variation of PR protein levels of [3H]R5020 binding activity. A parallel analysis of PR mRNA and [3H]R5020 binding was then performed in a series of tumor biopsies. In estrogen receptor-positive and PR-positive tissues a correlation among the two values was found. It is postulated that the above mentioned data could reflect the existence of a difference in the mechanisms controlling the numerous steps of the PR synthesis in the various hormone-responsive tissues. This variability could allow an organ-specific response to the cyclic changes of circulating hormone.
在对表达雌激素受体的T47D亚系进行的平行实验中，检测了孕酮受体（PR）mRNA、PR蛋白水平以及[3H]R5020结合活性。在细胞暴露于雌二醇（10^(-8) M）后6小时内，可检测到PR mRNA水平显著升高。然而，mRNA的变化并未导致PR蛋白水平或[3H]R5020结合活性发生任何变化。随后，在一系列肿瘤活检样本中对PR mRNA和[3H]R5020结合进行了平行分析。在雌激素受体阳性和PR阳性组织中，发现了这两个值之间的相关性。据推测，上述数据可能反映出在不同激素反应性组织中，控制PR合成众多步骤的机制存在差异。这种变异性可能允许器官对循环激素的周期性变化产生特异性反应。
