Saglio G, Foá R, Guerrasio A, di Celle P F, Palestro G, Sinicco A, Gobbi M, Gavosto F
Sezione de Clinica Medica, Departimento di Scienze Biomediche e Oncologia Umana, Torino, Italy.
Cancer Detect Prev. 1990;14(3):359-62.
The pattern of immunoglobulin (Ig) gene rearrangement and the genomic structure of the Myc locus were investigated in the DNA of 20 lymphnode biopsies of patients suffering from lymphoadenopathy-associated syndrome (LAS) and from 3 patients with Burkitt's lymphoma. Although polyclonality was the prevalent pattern of Ig gene rearrangement observed in LAS, in 30% of the cases discrete bands of Ig heavy chain gene rearrangement were identifiable due to the presence of monoclonal or oligoclonal cell populations. However, structural alterations of the Myc gene were not detected in any cases. As expected, in all three Burkitt's lymphomas studied, the lymphnode DNA displayed a clonal pattern of Ig heavy chain gene rearrangement. The Myc was altered in two cases, which presented a truncation of the gene beginning within a very short region of the first intron. By contrast, the breakpoint positions on chromosome 14 mapped in different regions of the Ig loci, which in both cases involved the switch (SH) area. Data confirm the relatively common occurrence of oligoclonal expansions within B cells in LAS and the frequent involvement of the Myc oncogene in the process of lymphomagenesis in individuals positive for human immunodeficiency virus (HIV).
在20例患有淋巴结病相关综合征（LAS）患者的淋巴结活检组织以及3例伯基特淋巴瘤患者的DNA中，研究了免疫球蛋白（Ig）基因重排模式和Myc基因座的基因组结构。尽管多克隆性是LAS中观察到的Ig基因重排的普遍模式，但在30%的病例中，由于存在单克隆或寡克隆细胞群体，可识别出Ig重链基因重排的离散条带。然而，在所有病例中均未检测到Myc基因的结构改变。正如预期的那样，在所研究的所有3例伯基特淋巴瘤中，淋巴结DNA显示出Ig重链基因重排的克隆模式。在2例病例中Myc基因发生改变，表现为基因在第一个内含子的非常短区域内开始截断。相比之下，14号染色体上的断点位置映射在Ig基因座的不同区域，在这两种情况下均涉及转换（SH）区域。数据证实LAS中B细胞内寡克隆扩增相对常见，并且Myc癌基因在人类免疫缺陷病毒（HIV）阳性个体的淋巴瘤发生过程中频繁受累。
