Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic, European Union.
Anticancer Agents Med Chem. 2014 Feb;14(2):331-40. doi: 10.2174/18715206113139990316.
Ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole) is an alkaloid that has been isolated from plants of an Apocynaceae family. It is one of the simplest naturally occurring alkaloids with a planar structure. Over the past decades, ellipticine became a very promising antitumor agent. Interaction with DNA is one of the most studied ellipticine effects on cell division. This phenomenon is not clearly explained so far. In our experiments we studied interaction of ellipticine with single-stranded and double-stranded oligonucleotides by electrochemical methods on mercury electrode. Differential pulse voltammetry was applied for ellipticine (Elli) and CA peak detection. Square wave voltammetry was applied for G peak detection. The effect of the interaction time and ellipticine concentrations on interactions of ellipticine with single- and double-stranded oligonucleotides was tested too.
椭圆精（5,11-二甲基-6H-吡啶并[4,3-b]咔唑）是一种生物碱，已从夹竹桃科植物中分离出来。它是具有平面结构的最简单的天然生物碱之一。在过去的几十年中，椭圆精已成为一种非常有前途的抗肿瘤药物。与 DNA 的相互作用是椭圆精对细胞分裂影响的最常见的研究之一。到目前为止，这一现象还没有得到明确的解释。在我们的实验中，我们使用电化学方法在汞电极上研究了椭圆精与单链和双链寡核苷酸的相互作用。差分脉冲伏安法用于椭圆精（Elli）和 CA 峰的检测。方波伏安法用于 G 峰的检测。还测试了相互作用时间和椭圆精浓度对椭圆精与单链和双链寡核苷酸相互作用的影响。
