Department of Infection, Immunity & Inflammation, University of Leicester, Maurice Shock Building, University Road, Leicester, LE1 9HN, United Kingdom; Department of Parasitology, Faculty of Medicine Building, University of Malaya, Kuala Lumpur, 50603, Malaysia.
J Eukaryot Microbiol. 2013 Sep-Oct;60(5):539-43. doi: 10.1111/jeu.12062. Epub 2013 Jul 19.
The free-living amoeba Balamuthia mandrillaris causes usually fatal encephalitis in humans and animals. Only limited studies have investigated the efficacy of antimicrobial agents against the organism. Assay methods were developed to assess antimicrobial efficacy against both the trophozoite and cyst stage of B. mandrillaris (ATCC 50209). Amphotericin B, ciclopirox olamine, miltefosine, natamycin, paromomycin, pentamidine isethionate, protriptyline, spiramycin, sulconazole and telithromycin had limited activity with amoebacidal levels of > 135-500 μM. However, diminazene aceturate (Berenil(®) ) was amoebacidal at 7.8 μM and 31.3-61.5 μM for trophozoites and cysts, respectively. Assays for antimicrobial testing may improve the prognosis for infection and aid in the development of primary selective culture isolation media.
自由生活的阿米巴原虫巴尔默氏阿米巴原虫通常会导致人类和动物致命性脑炎。只有有限的研究调查了对抗该生物体的抗菌药物的疗效。开发了检测方法来评估两性霉素 B、环吡酮胺、米替福新、纳他霉素、巴龙霉素、戊脒定、普罗替林、螺旋霉素、舒康唑和泰利霉素对巴尔默氏阿米巴原虫（ATCC 50209）的滋养体和囊包阶段的抗菌功效。两性霉素 B、环吡酮胺、米替福新、纳他霉素、巴龙霉素、戊脒定、普罗替林、螺旋霉素、舒康唑和泰利霉素对滋养体和囊包的杀菌水平 > 135-500 μM 时有有限的活性。然而，苯并咪唑乙酰脲（贝伦尼（®））对滋养体和囊包的杀菌水平分别为 7.8 μM 和 31.3-61.5 μM。抗菌药物检测的检测方法可以改善感染的预后，并有助于开发原发性选择性培养分离培养基。
