正畸力对大鼠牙周组织炎症改建及 IL-6、IL-8 表达的影响。

Effect of orthodontic force on inflammatory periodontal tissue remodeling and expression of IL-6 and IL-8 in rats.

机构信息

Department of Stomatology, First Affiliated Hospital of Zhengzhou University, No 1. the East Jianshe Road of Zhengzhou City, Henan Province 450000, China.

出版信息

Asian Pac J Trop Med. 2013 Oct;6(10):757-61. doi: 10.1016/S1995-7645(13)60133-9.

DOI:10.1016/S1995-7645(13)60133-9

PMID:23870461

Abstract

OBJECTIVE

To investigate effect of orthodontic force on inflammatory periodontal tissue remodeling and expression of IL-6 and IL-8 in rats.

METHODS

Eighty SD rats were randomly divided into 4 groups, blank control group (group A) with 5 rats, treatment normal group (group B) with 25 rats, inflammation control group (group (group C) with 25 rats, inflammation treatment group (group D) with 25 rats. Immunohistochemistry and histomorphometric analysis was performed to measure the expression of IL-6, IL-8 and the first molar to the recent movement in the distance.

RESULTS

The expression of IL-8 reached a maximum on day 5 and declined thereafter in group B; the expression of IL-6 reached a maximum on day 5 in group B. The expression of IL-6 and IL-8 was gradually weakened with time in group C. The expression of IL-6 and IL-8 were high, and reached a maximum on day 5 and declined thereafter in group D. AD of positive cells in group D were higher than group B at each time point (P<0.05). The time which 0.49 N orthodontic force was loaded was longer, orthodontic tooth movement distance was greater. Movement distance in group D were longer than group B (P<0.05).

CONCLUSIONS

Orthodontic force as well as inflammatory stimulus can evoke the expression of IL-6 and IL-8. Under the combined effects of inflammation and orthodontic force, the expression of IL-6, IL-8 will increase.

摘要

目的

研究正畸力对炎症性牙周组织重塑及白细胞介素-6（IL-6）和白细胞介素-8（IL-8）表达的影响。

方法

将 80 只 SD 大鼠随机分为 4 组，空白对照组（A 组）5 只，常规处理对照组（B 组）25 只，炎症对照组（C 组）25 只，炎症处理组（D 组）25 只。采用免疫组化和组织形态计量学分析测量 IL-6、IL-8 的表达及第一磨牙向近中移动的距离。

结果

B 组 IL-8 表达于第 5 天达高峰，此后逐渐下降；B 组 IL-6 表达于第 5 天达高峰。C 组随时间推移，IL-6 和 IL-8 表达逐渐减弱。D 组 IL-6 和 IL-8 表达较高，于第 5 天达高峰，此后逐渐下降。D 组各时间点阳性细胞 AD 值均高于 B 组（P<0.05）。0.49 N 正畸力加载时间越长，牙齿移动距离越大。D 组的移动距离大于 B 组（P<0.05）。