Department of Orthodontics, School of Stomatology, China Medical University, Liaoning, China.
Angle Orthod. 2009 Nov;79(6):1126-32. doi: 10.2319/100508-519R.1.
To investigate the changes of caspase-1 in orthodontic tooth movement and to determine whether the changes are phase-specific.
Eighty Wistar rats were included in this study. Sentalloy closed-coil springs were placed to induce a mesial traction force on the lower right first molar. The animals were killed after 1, 3, 7, and 14 days (n = 20 at each time point). The mandibles of 10 rats were sampled for histomorphometric analysis and immunohistochemical assay, and the periodontal tissues of 10 other rats were sampled for detecting caspase-1 mRNA and protein by real-time RT-PCR and by Western blotting, respectively.
The inflammatory reaction was evident in paraffin sections with hematoxylin-eosin staining. The immunohistochemical assay showed that orthodontic forces significantly increased the number of caspase-1-positive cells in the periodontal ligament (PDL). Mechanical force triggered an increase of caspase-1 mRNA in periodontal tissues. The expression of caspase-1 mRNA increased from day 1, reached the peak on day 3, and then decreased. The results of Western blotting indicated that the levels of both procaspase-1 and P20 subunit significantly increased after the application of orthodontic forces, compared with those in controls (P < .05).
Caspase-1 level increases during orthodontic tooth movement and changes with different phases, which might play a significant role in orthodontic tooth movement.
研究 caspase-1 在正畸牙齿移动中的变化，并确定这些变化是否具有时相特异性。
本研究纳入 80 只 Wistar 大鼠。在右侧下颌第一磨牙上放置 Sentalloy 封闭螺旋弹簧以产生近中牵引。在第 1、3、7 和 14 天（每个时间点 n = 20）处死动物。10 只大鼠的下颌骨用于组织形态计量学分析和免疫组织化学检测，另外 10 只大鼠的牙周组织用于通过实时 RT-PCR 和 Western 印迹分别检测 caspase-1 mRNA 和蛋白。
苏木精-伊红染色的石蜡切片显示出明显的炎症反应。免疫组织化学检测显示，正畸力显著增加了牙周韧带（PDL）中 caspase-1 阳性细胞的数量。机械力触发牙周组织中 caspase-1 mRNA 的增加。mRNA 的表达从第 1 天开始增加，在第 3 天达到峰值，然后下降。Western 印迹结果表明，与对照组相比，施加正畸力后，原 Caspase-1 和 P20 亚基的水平均显著增加（P <.05）。
caspase-1 水平在正畸牙齿移动过程中增加，并随不同阶段而变化，可能在正畸牙齿移动中发挥重要作用。
