Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Food Chem. 2013 Dec 1;141(3):2405-11. doi: 10.1016/j.foodchem.2013.05.037. Epub 2013 May 18.
Ferrous oxidation-xylenol orange assay reagent was reformulated by using spectral analysis of ferric-xylenol orange complex to detect low concentrations of lipoxygenase rice grain products. Reducing the levels of ferrous sulphate and xylenol orange in the FOX reagent enabled the detection of low concentrations of hydroperoxy fatty acid derived from lipoxygenase activity in the range of 0.1-1.5 μM. Protein, substrate and time courses of the modified FOX assay were studied to determine lipoxygenase activity in rice grain. The assay was also applicable as a high throughput technique for comparisons of lipoxygenase activity from various rice varieties. This has important implications for rapid screening for low-lipoxygenase containing rice cultivars in rice breeding program and grain quality during storage.
亚铁氧化-二甲酚橙测定试剂通过光谱分析铁-二甲酚橙络合物被重新配制，用于检测低浓度的脂氧合酶稻米产物。降低 FOX 试剂中硫酸亚铁和二甲酚橙的水平，使得能够检测到源于脂氧合酶活性的低浓度过氧脂肪酸，范围为 0.1-1.5 μM。研究了改良 FOX 测定法的蛋白质、底物和时间过程，以确定稻米中的脂氧合酶活性。该测定法也可作为一种高通量技术，用于比较不同水稻品种的脂氧合酶活性。这对于在水稻育种计划和储存期间快速筛选含低脂氧合酶的水稻品种以及谷物质量具有重要意义。
