Coordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6., Hermosillo, Sonora 83000, Mexico.
Food Chem. 2013 Dec 1;141(3):2727-34. doi: 10.1016/j.foodchem.2013.05.040. Epub 2013 May 21.
Enterotoxigenic (ETEC) Escherichia coli (E. coli) causes traveller's diarrhoea and high mortality among baby animals. ETEC adhesion is mediated by lectins (adhesins) that bind to glycoconjugates on the surface of host cells. Glycans that compete for adhesion could be used for disease prevention. Neoglycans of porcine albumin (PSA) that were conjugated with prebiotic galactooligosaccharides (GOS) were synthesised using the Maillard reaction. PSA glycation was confirmed by a reduction in the number of available free amino groups, decreased tryptophan intrinsic fluorescence, increased molecular mass and Ricinus communis lectin recognition. The adhesion of four ETEC strains (E. coli H10407, CFA(+), K99 and K88) to PSA-GOS was examined by an enzyme-linked lectin assay. E. coli K88 bound to PSA-GOS with greater affinity (P<0.05) than did E. coli H10407, CFA(+) and K99. In addition, PSA-GOS partially inhibited the adherence of the K88 strain to intestinal mucins. Pig ETEC strain was unable to ferment galactooligosaccharide-neoglycans. These results suggest that neoglycans obtained by the Maillard reaction may serve in the prophylaxis of ETEC K88 diarrhoea.
肠产毒性（ETEC）大肠杆菌（E. coli）可引起旅行者腹泻和幼小动物的高死亡率。ETEC 的黏附是由与宿主细胞表面糖缀合物结合的凝集素（黏附素）介导的。竞争黏附的聚糖可用于疾病预防。使用美拉德反应合成了与益生元半乳糖寡糖（GOS）缀合的猪白蛋白（PSA）的新糖。通过减少可用游离氨基基团的数量、降低色氨酸固有荧光、增加分子量和蓖麻凝集素识别来确认 PSA 糖基化。通过酶联凝集素测定法检查了四种 ETEC 菌株（大肠杆菌 H10407、CFA(+)、K99 和 K88）与 PSA-GOS 的黏附。与大肠杆菌 H10407、CFA(+)和 K99 相比，大肠杆菌 K88 与 PSA-GOS 的结合亲和力更强（P<0.05）。此外，PSA-GOS 部分抑制了 K88 菌株对肠粘蛋白的黏附。猪 ETEC 菌株不能发酵半乳糖寡糖-新糖。这些结果表明，美拉德反应获得的新糖可能用于预防 ETEC K88 腹泻。
