Molecular Biology Unit, CeDReS, University Hospital of Treichville, Abidjan, Cote d'Ivoire; University Felix Houphouet Biogny of Cocody, Abidjan, Cote d'Ivoire.
J Virol Methods. 2013 Nov;193(2):439-45. doi: 10.1016/j.jviromet.2013.07.011. Epub 2013 Jul 16.
This study evaluates HIV infant diagnosis on DBS using Biocentric HIV1-DNA and HIV1-RNA assays, in field conditions in Côte d'Ivoire. Paediatric screening was offered to children≤3 years in clinical sites in Côte d'Ivoire in 2008. For each HIV-infected child, two non-infected children were included and blood samples were collected. HIV-DNA results obtained on EDTA blood samples with Biocentric assay were the reference for HIV infant diagnosis. Plasma and DBS viral loads were measured using HIV-RNA Biocentric assay. DBS samples were also tested for HIV-DNA detection using both Biocentric and Amplicor Roche assays. Sensitivity, specificity and concordance between tests were calculated. Overall samples from 138 HIV-exposed children, 46 infected, 92 non-infected were included. All tests were 100% sensitive and specific including 100% concordance with the two HIV-DNA assays. The median level of HIV-DNA on EDTA samples was 3.15 log10 copies/10(6) PBMCs; the median level of HIV RNA in plasma and DBS were respectively 5.82 and 5.17 log10 copies/ml (Pearson's correlation R2=0.92, p<0.0001). The threshold for detectable HIV-RNA on DBS was 3.3 log10. Although there are differences between viral load measured on DBS and plasma, the two Biocentric assays present very good performances for HIV infant diagnosis on DBS while cheap and feasible.
本研究评估了 Biocentric HIV1-DNA 和 HIV1-RNA 检测试剂盒在科特迪瓦现场条件下用于干血斑（DBS）的 HIV 婴儿诊断。2008 年，在科特迪瓦的临床点为≤3 岁的儿童提供了儿科筛查。对于每例 HIV 感染的儿童，纳入 2 名非感染儿童，并采集血样。使用 Biocentric 试剂盒从 EDTA 血样中获得的 HIV-DNA 结果被用作 HIV 婴儿诊断的参考。使用 HIV-RNA Biocentric 检测试剂盒测量血浆和 DBS 病毒载量。还使用 Biocentric 和 Amplicor Roche 检测试剂盒检测 DBS 样本中 HIV-DNA 的检测。计算了测试之间的敏感性、特异性和一致性。共纳入了 138 名 HIV 暴露儿童、46 名感染儿童和 92 名未感染儿童的样本。所有测试均具有 100%的敏感性和特异性，包括与两种 HIV-DNA 检测试剂盒的 100%一致性。EDTA 样本中 HIV-DNA 的中位数水平为 3.15 log10 拷贝/10(6) PBMC；血浆和 DBS 中 HIV RNA 的中位数水平分别为 5.82 和 5.17 log10 拷贝/ml（Pearson 相关系数 R2=0.92，p<0.0001）。DBS 上可检测到 HIV-RNA 的阈值为 3.3 log10。尽管 DBS 和血浆上测量的病毒载量存在差异，但两种 Biocentric 检测试剂盒在 DBS 上用于 HIV 婴儿诊断时具有非常好的性能，而且便宜且可行。
