Kojima M K, Czihak G K
Department of Biology, Faculty of Science, Toyama University, Japan.
Eur J Cell Biol. 1990 Jun;52(1):129-34.
Eggs of Paracentrotus exposed to 1 mM 2,4-dinitrophenol (DNP) in sea water at 10-min intervals from 10 to 40 min after insemination and returned to normal sea water 60 min later, divide into two cells. If, however, such eggs are treated for 60 min beginning at 50 min after insemination, they cleave directly into four cells. Division centers can split and separate in the DNP solution though the asters appear very faint and the spindle considerably reduced in size. This means that DNP has an inhibitory effect on duplication of division centers but not on the splitting and separation of centers already duplicated. Cytophotometric comparison of the DNA content in di-, tri- and tetrapolar mitosis of DNP-treated and control eggs revealed no difference showing that chromosome cycle and centrosome cycle are independent events in the cell cycle.
将受精后10至40分钟的海胆卵每隔10分钟暴露于1 mM 2,4-二硝基苯酚（DNP）海水中，60分钟后再放回正常海水中，这些卵会分裂为两个细胞。然而，如果在受精后50分钟开始将此类卵处理60分钟，它们会直接分裂为四个细胞。尽管星体显得非常模糊且纺锤体大小显著减小，但在DNP溶液中分裂中心仍可分裂和分离。这意味着DNP对分裂中心的复制有抑制作用，但对已复制中心的分裂和分离没有影响。对经DNP处理的卵和对照卵的二极、三极和四极有丝分裂中的DNA含量进行细胞光度测定比较，结果显示没有差异，这表明染色体周期和中心体周期在细胞周期中是独立事件。
