Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyangsi, Gyeonggido, South Korea.
Poult Sci. 2013 Aug;92(8):2053-8. doi: 10.3382/ps.2013-03134.
This study was conducted to characterize infectious laryngotracheitis (ILT) viruses isolated from poultry in South Korea using RFLP analysis of PCR products. Seven wild-type Korean isolates from commercial chicken farms collected between 1986 and 2012 were compared with 3 imported commercial vaccine strains [LT Blen (Hudson strain, United States), Laryngo Vac (Cover strain, United States), and Nobilis ILT (Serva strain, France)] and a Korean chicken embryo origin (CEO) vaccine strain [ILT-VAC (Gyeonggi97 strain, Korea)]. Six of the field isolates were highly virulent viruses, and the Kr12AD37 isolate was considered an attenuated type according to Han's RFLP method. These virulent Korean ILT viruses were divided into 3 classes (class I, II, and III). The Kr12AD37 isolate was found to have the same RFLP pattern as the Korean CEO vaccine strain, and both of these strains were different from the 3 foreign vaccine strains. The results suggest that the Korean CEO vaccine strain has been responsible for recent outbreaks, and the characterization of ILT viruses by RFLP was useful for diagnosis by providing epidemiological information.
本研究采用 PCR-RFLP 分析方法对韩国禽源传染性喉气管炎（ILT）病毒进行了鉴定。将 1986 年至 2012 年间从商业养鸡场采集的 7 株野毒株与 3 株进口商业疫苗株（LT Blen（美国哈德逊株）、Laryngo Vac（美国科威尔株）和 Nobilis ILT（法国赛尔福株））和 1 株韩国鸡胚源（CEO）疫苗株（ILT-VAC（韩国京畿道 97 株））进行了比较。其中 6 株分离株为高致病性病毒，根据 Han 的 RFLP 方法，Kr12AD37 分离株被认为是弱毒型。这些高致病性韩国 ILT 病毒分为 3 个型（I 型、II 型和 III 型）。Kr12AD37 分离株与韩国 CEO 疫苗株具有相同的 RFLP 图谱，这两种毒株均与 3 株国外疫苗株不同。结果表明，韩国 CEO 疫苗株可能是近期暴发的原因，通过 RFLP 对 ILT 病毒进行特征分析，为提供流行病学信息，有助于对其进行诊断。
