Dipartimento di Chimica e Farmacia, Università degli Studi di Sassari, Sassari, Italy.
Chirality. 2013 Oct;25(10):617-21. doi: 10.1002/chir.22185. Epub 2013 Jul 22.
An easy-to-prepare chiral CE method for the enantiomeric separation of 13 new amphetamine-like designer drugs, using CDs as chiral selectors, was developed. Sulfated-β-CD was found to be the best chiral selector among the three used (sulfated-β-CD, caroboxymethyl-β-CD, dimethyl-β-CD). The separation of the analytes was achieved in a fused-silica gel capillary at 20 °C using an applied voltage of +25 kV. The optimized background electrolyte consisted of 63.5 mM H3 PO4 and 46.9 mM NaOH in water. Several electrophoretic parameters such as CD type, CD concentration (1 - 40 mg/mL), buffer pH (2.6, 3.6, 5.0, 6.0), length of the capillary (70 - 40 cm total length), amount of the organic solvent (methanol and acetonitrile) were investigated and optimized.
开发了一种使用 CDs 作为手性选择剂，易于制备的手性 CE 方法，用于对 13 种新型苯丙胺类设计药物的对映体分离。在三种使用的手性选择剂（硫酸化-β-CD、羧甲基-β-CD、二甲基-β-CD）中，硫酸化-β-CD 被发现是最佳的手性选择剂。在 20°C 下，使用+25 kV 的外加电压，在熔融石英毛细管中实现了分析物的分离。优化后的背景电解质由水中的 63.5 mM H3PO4 和 46.9 mM NaOH 组成。研究并优化了几种电泳参数，如 CD 类型、CD 浓度（1-40 mg/mL）、缓冲液 pH 值（2.6、3.6、5.0、6.0）、毛细管长度（总长度 70-40 cm）、有机溶剂（甲醇和乙腈）的量。
