硅基上精细圆柱形玻璃微毛细管的整体集成用于生物分子的电泳分离。
Monolithic integration of fine cylindrical glass microcapillaries on silicon for electrophoretic separation of biomolecules.
机构信息
Department of Electronic and Computer Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
出版信息
Biomicrofluidics. 2012 Jul 20;6(3):36501. doi: 10.1063/1.4739075. Print 2012 Sep.
We demonstrate monolithic integration of fine cylindrical glass microcapillaries (diameter ∼1 μm) on silicon and evaluate their performance for electrophoretic separation of biomolecules. Such microcapillaries are achieved through thermal reflow of a glass layer on microstructured silicon whereby slender voids are moulded into cylindrical tubes. The process allows self-enclosed microcapillaries with a uniform profile. A simplified method is also described to integrate the microcapillaries with a sample-injection cross without the requirement of glass etching. The 10-mm-long microcapillaries sustain field intensities up to 90 kV/m and limit the temperature excursions due to Joule heating to a few degrees Celsius only.
我们展示了精细圆柱形玻璃微毛细管（直径约 1μm）在硅片上的整体集成，并评估了它们在生物分子电泳分离方面的性能。这种微毛细管是通过在微结构硅片上的玻璃层进行热回流来实现的，在此过程中，细长的空隙被模制成圆柱形管。该工艺允许自封闭的微毛细管具有均匀的轮廓。还描述了一种简化的方法来将微毛细管与样品注入交叉集成，而无需进行玻璃蚀刻。10 毫米长的微毛细管可承受高达 90kV/m 的场强，并且由于焦耳加热引起的温度变化仅局限在几度范围内。
Zotero 插件