Institute for Biochemistry and Molecular Biology, Ulm University, Ulm, Germany.
PLoS One. 2013 Jul 18;8(7):e69372. doi: 10.1371/journal.pone.0069372. Print 2013.
SoxC genes are involved in many developmental processes such as cardiac, lymphoid, and bone development. The SoxC gene family is represented by Sox4, Sox11, and Sox12. Loss of either Sox4 or Sox11 function is lethal during mouse embryogenesis. Here, we demonstrate that sox4 and sox11 are strongly expressed in the developing eye, heart as well as brain in Xenopus laevis. Morpholino oligonucleotide mediated knock-down approaches in anterior neural tissue revealed that interference with either Sox4 or Sox11 function affects eye development. A detailed analysis demonstrated strong effects on eye size and retinal lamination. Neural induction was unaffected upon Sox4 or Sox11 MO injection and early eye field differentiation and cell proliferation were only mildly affected. Depletion of both genes, however, led independently to a significant increase in cell apoptosis in the eye. In summary, Sox4 and Sox11 are required for Xenopus visual system development.
SoxC 基因参与许多发育过程，如心脏、淋巴样和骨骼发育。 SoxC 基因家族由 Sox4、Sox11 和 Sox12 代表。在小鼠胚胎发生过程中，Sox4 或 Sox11 功能的丧失是致命的。在这里，我们证明 Sox4 和 Sox11 在非洲爪蟾的发育眼睛、心脏和大脑中强烈表达。在前神经组织中，通过 morpholino 寡核苷酸介导的敲低方法，发现干扰 Sox4 或 Sox11 的功能会影响眼睛发育。详细分析表明，对眼睛大小和视网膜分层有强烈影响。 Sox4 或 Sox11 MO 注射对神经诱导没有影响，早期眼区分化和细胞增殖也只有轻微影响。然而，这两种基因的缺失都会导致眼睛中的细胞凋亡显著增加。总之，Sox4 和 Sox11 是非洲爪蟾视觉系统发育所必需的。
