Huang Sheng-Ping, Huang Chun-Hsiang, Shyu Jia-Fwu, Lee Herng-Sheng, Chen Shyi-Gen, Chan James Yi-Hsin, Huang Shih-Ming
J Biomed Sci. 2013 Jul 22;20(1):51. doi: 10.1186/1423-0127-20-51.
Wound healing is a complex biologic process that involves the integration of inflammation, mitosis, angiogenesis, synthesis, and remodeling of the extracellular matrix. However, some wounds fail to heal properly and become chronic. Although some simulated chronic wound models have been established, an efficient approach to treat chronic wounds in animal models has not been determined. The aim of this study was to develop a modified rat model simulating the chronic wounds caused by clinical radiation ulcers and examine the treatment of chronic wounds with adipose-derived stem cells.
Sprague-Dawley rats were irradiated with an electron beam, and wounds were created. The rats received treatment with adipose-derived stem cells (ASCs), and a wound-healing assay was performed. The wound sizes after ASC treatment for 3 weeks were significantly smaller compared with the control condition (p < 0.01). Histological observations of the wound edge and immunoblot analysis of the re-epithelialization region both indicated that the treatment with ASCs was associated with the development of new blood vessels. Cell-tracking experiments showed that ASCs were colocalized with endothelial cell markers in ulcerated tissues.
We established a modified rat model of radiation-induced wounds and demonstrated that ASCs accelerate wound-healing.
伤口愈合是一个复杂的生物学过程，涉及炎症、有丝分裂、血管生成、细胞外基质的合成与重塑。然而，一些伤口无法正常愈合而变成慢性伤口。尽管已经建立了一些模拟慢性伤口的模型，但尚未确定在动物模型中治疗慢性伤口的有效方法。本研究的目的是建立一种改良的大鼠模型，模拟临床放射性溃疡引起的慢性伤口，并研究脂肪干细胞对慢性伤口的治疗作用。
用电子束照射斯普拉格-道利大鼠并造成伤口。给大鼠注射脂肪干细胞（ASC）进行治疗，并进行伤口愈合试验。与对照相比，ASC治疗3周后的伤口大小明显更小（p < 0.01）。伤口边缘的组织学观察和再上皮化区域的免疫印迹分析均表明，ASC治疗与新血管的形成有关。细胞追踪实验表明，ASC与溃疡组织中的内皮细胞标志物共定位。
我们建立了一种改良的放射性伤口大鼠模型，并证明ASC可加速伤口愈合。
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