Talbot J A, Shalet S M, Tsatsoulis A, Grabinski M, Robertson W R
Department of Medicine (Clinical Biochemistry), University of Manchester, Hope Hospital, Salford, U.K.
Int J Androl. 1990 Jun;13(3):223-31. doi: 10.1111/j.1365-2605.1990.tb00980.x.
Bioactive-LH (B-LH) was measured in plasma by in-vitro bioassay and immunoactive-LH (I-LH) by immunoassay at 10 min intervals for 6 h in five men after standard chemotherapy for Hodgkin's disease. Eleven normal men acted as controls. Follicle-stimulating hormone (FSH) was markedly raised in the treated patients (mean +/- SEM; 12.8 +/- 2.8 vs. 2.7 +/- 0.4 IU l-1, P less than 0.006) reflecting damage to the germinal epithelium. Bioactive (27.4 +/- 2.8 vs. 12.9 +/- 1.3 IU l-1) and I-LH (9.6 +/- 2.0 vs. 4.9 +/- 0.4 IU l-1) were elevated (P less than 0.006) in the patient group whilst testosterone levels (24.0 +/- 3.8 vs. 19.6 +/- 2.4 nmol l-1) were normal. The testosterone I-LH ratio, a putative index of Leydig cell dysfunction, was negatively correlated with FSH levels (r = -0.85, P less than 0.02). Bioactive and I-LH pulse peak amplitude were elevated, as were pulse maxima (P less than 0.05). In contrast, B-LH pulse frequency was similar between the patients (2 pulses per 6 h) and controls (median 2, range 1-3 pulses per 6 h) as was the I-LH pulse frequency (median 2, 1-2 pulses per 6 h in both groups). The mean B:I LH ratios were similar (2.94 +/- 0.09 vs. 2.63 +/- 0.14) in both groups, although the inter-pulse B:I ratio was increased (P less than 0.007) in the patient group.(ABSTRACT TRUNCATED AT 250 WORDS)
对5名接受霍奇金病标准化疗后的男性，每隔10分钟测定一次血浆中的生物活性促黄体生成素（B-LH）（采用体外生物测定法）和免疫活性促黄体生成素（I-LH）（采用免疫测定法），持续6小时。11名正常男性作为对照。治疗后的患者促卵泡生成素（FSH）显著升高（均值±标准误；12.8±2.8对比2.7±0.4 IU l-1，P＜0.006），反映出生精上皮受损。患者组的生物活性促黄体生成素（27.4±2.8对比12.9±1.3 IU l-1）和I-LH（9.6±2.0对比4.9±0.4 IU l-1）升高（P＜0.006），而睾酮水平（24.0±3.8对比19.6±2.4 nmol l-1）正常。睾酮与I-LH的比值是推测的睾丸间质细胞功能障碍指标，与FSH水平呈负相关（r = -0.85，P＜0.02）。生物活性促黄体生成素和I-LH的脉冲峰值幅度升高，脉冲最大值也升高（P＜0.05）。相比之下，患者组（每6小时2次脉冲）和对照组（中位数为2次，每6小时1 - 3次脉冲）的B-LH脉冲频率相似，I-LH脉冲频率也相似（两组中位数均为2次，每6小时1 - 2次脉冲）。两组的平均B:I LH比值相似（2.94±0.09对比2.63±0.14），尽管患者组脉冲间期的B:I比值升高（P＜0.007）。（摘要截断于250字）
