New Materials Division, FCBA, Intechfibres, Domaine Universitaire, BP 251, F-38044 Grenoble Cedex 9, France.
J Biotechnol. 2013 Sep 10;167(3):302-8. doi: 10.1016/j.jbiotec.2013.07.014. Epub 2013 Jul 19.
Hydrophobic properties were conferred to a high-lignin-content Kraft pulp by a laccase-catalysed treatment in the presence of lauryl gallate (LG). The treatment resulted in a two-fold increase in contact angle and conferred water absorption resistance to the pulp. Kappa number was increased, indicating that some phenolic compounds were incorporated in the pulp. A control treatment with LG alone did not affect water absorption, demonstrating that laccase was essential to attain these new properties. The loss of hydrophobicity after an acetone Soxhlet extraction highlighted that adsorbed acetone-soluble compounds played a key role in the properties. GC-FID and HPSEC-UV analysis of the acetone extract indicated the formation of dodecanol and different phenolic oligomers. SEM images showed the treatment-induced changes in the fibre network. Additional experiments with various reaction times and reactant concentrations highlighted the role of LG oxidation products in the introduction of absorption resistance.
漆酶在没食子酸月桂酯（LG）存在下催化处理高木质素含量的 Kraft 浆，赋予其疏水性。处理后接触角增加了一倍，使纸浆具有抗吸水性。卡伯值增加，表明一些酚类化合物被结合到纸浆中。单独用 LG 进行的对照处理对吸水性没有影响，表明漆酶对于获得这些新性能是必不可少的。用丙酮索氏提取后疏水性的丧失突出表明，吸附的丙酮可溶化合物在这些性能中起着关键作用。丙酮提取物的 GC-FID 和 HPSEC-UV 分析表明形成了十二烷醇和不同的酚类低聚物。SEM 图像显示了纤维网络在处理过程中的变化。用不同的反应时间和反应物浓度进行的其他实验突出了 LG 氧化产物在引入抗吸收性方面的作用。
