Tuft S, al-Dhahir R, Dyer P, Zhu Z H
Moorfields Eye Hospital, London, United Kingdom.
Invest Ophthalmol Vis Sci. 1990 Aug;31(8):1512-8.
The epithelium and stroma of bovine and human corneas were exposed to both ablative and subablative levels of 193-nm excimer laser radiation and the spectra of the induced fluorescence recorded. Two broad peaks in the emission spectra were observed, at 310 nm and 460 nm, with a difference in the relative height of these peaks between epithelium and stroma. The lower cut-off for fluorescence (260 nm) was similar for both tissues. Time-resolved measurements showed that fluorescence occurs on the nanosecond time scale, and an estimate gives the quantum yield for photons emitted in the 260-350-nm range as approximately 1 X 10(-5). The significance of these results in the evaluation of the safety of surgery with an argon fluoride excimer laser is discussed.
将牛和人角膜的上皮和基质暴露于193纳米准分子激光的消融和亚消融水平，并记录诱导荧光的光谱。在发射光谱中观察到两个宽峰，分别位于310纳米和460纳米处，上皮和基质中这些峰的相对高度存在差异。两种组织的荧光下限（260纳米）相似。时间分辨测量表明，荧光发生在纳秒时间尺度上，估计在260-350纳米范围内发射的光子的量子产率约为1×10^(-5)。讨论了这些结果在评估氟化氩准分子激光手术安全性方面的意义。
