壳聚糖上溶菌酶的分离

Isolation of lysozyme on chitosan.

作者信息

Muzzarelli R A, Barontini G, Rocchetti R

出版信息

Biotechnol Bioeng. 1978 Jan;20(1):87-94. doi: 10.1002/bit.260200107.

DOI:10.1002/bit.260200107

Abstract

Lysozyme has been immobilized on chitosan, a polyaminosaccharide, without using any intermediate reagent. The best pH conditions for operating the chitosan columns have been determined and the best eluting agent was found to be a 2% solution of propylamine. The lysozyme activity was determined after reacting lysozyme with the product of glycolchitin and Remazol Brilliant Blue R. The recovery of lysozyme from chicken egg white yields lysozyme with 55% activity.

摘要

溶菌酶已被固定在壳聚糖（一种聚氨基糖）上，且未使用任何中间试剂。已确定操作壳聚糖柱的最佳pH条件，并且发现最佳洗脱剂是2%的丙胺溶液。在溶菌酶与乙二醇壳聚糖和活性艳蓝R的产物反应后测定了溶菌酶活性。从鸡蛋白中回收的溶菌酶活性为55%。