Vitaioli L, Baldoni E, Bellini L, Bolognani L
Department of Cellular Biology, University of Camerino, Italy.
Histochem J. 1990 Apr;22(4):192-6. doi: 10.1007/BF02386004.
Sulphatides have been studied by histochemical and biochemical procedures in the oviduct of the frog in different experimental conditions. In ovariectomized or hypophysectomized animals, compared to sham-operated, an increase in sulphatides was observed. The progesterone treatment did not significantly modify this lipid fraction in ovariectomized frogs, while in hypophysectomized frogs it induced a further increase. Densitographic profiles of the sulphatides, obtained by thin-layer chromatography (TLC) and also recorded by Tesak equipment, were similar in ovariectomized or hypophysectomized frogs following hormone treatment because they showed three distinct fractions in both experimental groups of animals. The appearance of a third fraction never previously observed was probably induced by the progesterone treatment. Moreover, under the effects of this hormone, the phospholipid fractions (phosphatidylcholine and phosphatidylethanolamine) also showed different densitographic profiles.
已通过组织化学和生化方法，在不同实验条件下对青蛙输卵管中的硫脂进行了研究。与假手术动物相比，在去卵巢或垂体切除的动物中，观察到硫脂增加。孕酮处理并未显著改变去卵巢青蛙的这种脂质成分，而在垂体切除的青蛙中，它导致了进一步增加。通过薄层色谱法（TLC）获得并由Tesak设备记录的硫脂光密度图谱，在激素处理后的去卵巢或垂体切除青蛙中相似，因为在两组实验动物中它们都显示出三个不同的组分。先前从未观察到的第三个组分的出现可能是由孕酮处理诱导的。此外，在这种激素的作用下，磷脂组分（磷脂酰胆碱和磷脂酰乙醇胺）也显示出不同的光密度图谱。
