Salari H, Eigendorf G K
Department of Medicine, University of British Columbia, Vancouver, Canada.
J Chromatogr. 1990 May 18;527(2):303-14. doi: 10.1016/s0378-4347(00)82114-3.
Lyso-platelet-activating factor (lyso-PAF) was derivatised with 9-anthracenepropionic acid in the presence of dicyclohexylcarbodiimide, p-toluenesulfonic acid and 4-dimethylaminopyridine. The reaction yield exceeded 90% when the fatty acid was present in double molar amounts versus lyso-PAF. The procedure was equally effective in the derivatisation of other lysophospholipids. The derivatized phospholipids are detected by ultraviolet absorption (lambda = 253 nm) or fluorescence detection (using excitation at 254 nm and emission at 450 nm). The technique was applied successfully to the detection of lyso-PAF in complement activated rabbit plasma.
溶血血小板活化因子（lyso-PAF）在二环己基碳二亚胺、对甲苯磺酸和4-二甲氨基吡啶存在的情况下与9-蒽丙酸进行衍生化反应。当脂肪酸与溶血血小板活化因子的摩尔比为2时，反应产率超过90%。该方法对其他溶血磷脂的衍生化同样有效。衍生化的磷脂通过紫外吸收（波长=253nm）或荧光检测（激发波长为254nm，发射波长为450nm）进行检测。该技术已成功应用于补体激活的兔血浆中溶血血小板活化因子的检测。
