Shi Yuting, Huang Fengzhen, Tang Beisha, Li Jiada, Wang Junling, Shen Lu, Xia Kun, Jiang Hong
1Department of Neurology, Xiangya Hospital, Central South University , Changsha, Hunan , China.
Int J Neurosci. 2014 Feb;124(2):97-101. doi: 10.3109/00207454.2013.827679. Epub 2013 Aug 22.
Spinocerebellar ataxia type 3/Machado-Joseph disease (SCA3/MJD) is the most common type of spinocerebellar ataxia in China. However, the pathogenesis of SCA3/MJD is still unknown. MicroRNAs (miRNAs) have been repeatedly demonstrated to exist in human peripheral serum in a bio-stable form and have been shown to be useful biomarkers for other neurodegenerative disorders. However, no study of SCA3/MJD patients has assessed specific changes in regulatory miRNAs. Therefore, we systematically used the miRCURYTM LNA Array, followed by quantitative real-time polymerase chain reaction validation, to access miRNA expression levels in the serums from SCA3/MJD patients. Our results show that miR-25, miR-125b, miR-29a, and miR-34b could be potential biomarkers for SCA3/MJD and could be used to further investigate the pathogenesis of SCA3/MJD and drug development.
3型脊髓小脑共济失调/马查多-约瑟夫病（SCA3/MJD）是中国最常见的脊髓小脑共济失调类型。然而，SCA3/MJD的发病机制仍不清楚。微小RNA（miRNA）已被反复证明以生物稳定的形式存在于人类外周血清中，并已被证明是其他神经退行性疾病的有用生物标志物。然而，尚无针对SCA3/MJD患者的研究评估调节性miRNA的具体变化。因此，我们系统地使用了miRCURYTM LNA芯片，随后进行定量实时聚合酶链反应验证，以检测SCA3/MJD患者血清中的miRNA表达水平。我们的结果表明，miR-25、miR-125b、miR-29a和miR-34b可能是SCA3/MJD的潜在生物标志物，可用于进一步研究SCA3/MJD的发病机制和药物开发。
