Han Bing, Wang Xiaoyan, Liu Jiguang, Liang Fuxin, Qu Xiaozhong, Yang Zhenzhong, Gao Xuejun
Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Beijing, China.
J Endod. 2013 Aug;39(8):1030-4. doi: 10.1016/j.joen.2013.04.014. Epub 2013 May 23.
Calcium hydroxide (Ca[OH]2) microcapsules were synthesized for use in controlled release. The aim of this study was to evaluate the cytotoxicity, antibacterial properties, and influence on gene expression of bone-related markers of 2 different formulas of Ca(OH)2 microcapsules.
Two formulas of Ca(OH)2 microcapsules (A and B) were evaluated, and pure Ca(OH)2 powder was used as a positive control. The shell material of formula A was pure EC, and the PLA/EC blend of 1:1 was used as the shell material for formula B. The MG63 cells/Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) were used to evaluate the cytotoxicity, and the colony-forming units of Enterococcus faecalis were monitored for the antibacterial effect. The relative messenger RNA expression of collagen I and osteocalcin was determined by real-time polymerase chain reaction.
Both formulas of the Ca(OH)2 microcapsules showed no cytotoxicity in MG63 cells; however, the Ca(OH)2 positive control did exhibit cytotoxicity. The antibacterial effect of the 2 microcapsule formulas lasted longer than the positive control, and formula A lasted longer than formula B. For both Ca(OH)2 microcapsule formulas, the relative messenger RNA expression of collagen I and osteocalcin was prolonged and up-regulated. The time effects of the influence on messenger RNA expression of collagen I and osteocalcin were different between the 2 microcapsule formulas.
Ca(OH)2 microcapsules had prolonged antibacterial activity and prolonged the up-regulation of bone-related markers with reduced cytotoxicity.
合成了用于控释的氢氧化钙（Ca[OH]₂）微胶囊。本研究的目的是评估两种不同配方的Ca(OH)₂微胶囊的细胞毒性、抗菌性能以及对骨相关标志物基因表达的影响。
评估了两种配方的Ca(OH)₂微胶囊（A和B），并使用纯Ca(OH)₂粉末作为阳性对照。配方A的壳材料为纯乙基纤维素（EC），配方B的壳材料为1:1的聚乳酸（PLA）/EC混合物。使用MG63细胞/细胞计数试剂盒-8（日本熊本同仁化学研究所）评估细胞毒性，并监测粪肠球菌的菌落形成单位以评估抗菌效果。通过实时聚合酶链反应测定I型胶原蛋白和骨钙素的相对信使核糖核酸（mRNA）表达。
两种配方的Ca(OH)₂微胶囊在MG63细胞中均未显示细胞毒性；然而，Ca(OH)₂阳性对照确实表现出细胞毒性。两种微胶囊配方的抗菌效果持续时间均长于阳性对照，且配方A的持续时间长于配方B。对于两种Ca(OH)₂微胶囊配方，I型胶原蛋白和骨钙素的相对mRNA表达均延长且上调。两种微胶囊配方对I型胶原蛋白和骨钙素mRNA表达影响的时间效应不同。
Ca(OH)₂微胶囊具有延长的抗菌活性，延长了骨相关标志物的上调，且细胞毒性降低。
