a Department of Pharmaceutical Analysis , Sir C. R. Reddy College of Pharmaceutical Sciences , Eluru , Andhra Pradesh , India.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2013;30(10):1739-44. doi: 10.1080/19440049.2013.816896. Epub 2013 Jul 24.
A new microwave-assisted derivatisation and LC-MS/MS method has been developed for the analysis of nitrofuran metabolites - 3-amino-5-morpholino-methyl-1,3-oxa-zolidinone (AMOZ), 3-amino-2-oxazolidinone (AOZ), 1-aminohydantoin (AHD) and semicarbazide (SEM) - in farm-raised prawns (Penaeus monodon) from the coastal regions of South India. Analysis was carried out by reverse-phase column (Phenomenex Luna C18) with gradient elution using mobile phase A (0.02% acetic acid in water) and mobile phase B (0.02% acetic acid in acetonitrile), at a flow rate of 200 μl min(-1) and an injection volume of 20 μl. Microwave-assisted derivatisation was achieved in 6 min with good recovery. The results showed that the samples collected from Andhra Pradesh and Karnataka contained residues of nitrofuran metabolites in the range from 5.0 to 40 ng g(-1). This work emphasises the importance of ensuring the safety of seafood and that a new method of derivatisation is applicable for the analysis of nitrofuran metabolites in seafood.
一种新的微波辅助衍生化和 LC-MS/MS 方法已被开发用于分析来自印度南部沿海地区养殖对虾（Penaeus monodon）中的硝基呋喃代谢物 - 3-氨基-5-吗啉基甲基-1,3-恶唑烷酮（AMOZ）、3-氨基-2-恶唑烷酮（AOZ）、1-氨基脲（AHD）和氨基脲（SEM）。分析采用反相柱（Phenomenex Luna C18），梯度洗脱，流动相 A（水中 0.02% 乙酸）和流动相 B（乙腈中 0.02% 乙酸），流速为 200 μl min(-1)，进样体积为 20 μl。微波辅助衍生化在 6 分钟内完成，回收率良好。结果表明，来自安得拉邦和卡纳塔克邦的样品中含有硝基呋喃代谢物残留，范围为 5.0 至 40 ng g(-1)。这项工作强调了确保海鲜安全的重要性，并且衍生化的新方法适用于海鲜中硝基呋喃代谢物的分析。
