Armour Natalie K, Laibinis Victoria A, Collett Stephen R, Ferguson-Noel Naola
a Department of Population Health, Poultry Diagnostic and Research Center , College of Veterinary Medicine, University of Georgia , Athens , Georgia , USA.
Avian Pathol. 2013;42(5):408-15. doi: 10.1080/03079457.2013.819486. Epub 2013 Jul 26.
Molecular analysis was conducted on 36 Mycoplasma gallisepticum DNA extracts from tracheal swab samples of commercial poultry in seven South African provinces between 2009 and 2012. Twelve unique M. gallisepticum genotypes were identified by polymerase chain reaction and sequence analysis of the 16S-23S rRNA intergenic spacer region (IGSR), M. gallisepticum cytadhesin 2 (mgc2), MGA_0319 and gapA genetic regions. The DNA sequences of these genotypes were distinct from those of M. gallisepticum isolates in a database composed of sequences from other countries, vaccine and reference strains. The most prevalent genotype (SA-WT#7) was detected in samples from commercial broilers, broiler breeders and layers in five provinces. South African M. gallisepticum sequences were more similar to those of the live vaccines commercially available in South Africa, but were distinct from that of F strain vaccine, which is not registered for use in South Africa. The IGSR, mgc2 or MGA_0319 sequences of three South African genotypes were identical to those of the ts-11 vaccine strain, necessitating a combination of mgc2 and IGSR targeted sequencing to differentiate South African wild-type genotypes from ts-11 vaccine. To identify and differentiate all 12 wild-types, mgc2, IGSR and MGA_0319 sequencing was required. Sequencing of gapA was least effective at strain differentiation. This research serves as a model for the development of an M. gallisepticum sequence database, and illustrates its application to characterize M. gallisepticum genotypes, select diagnostic tests and better understand the epidemiology of M. gallisepticum.
2009年至2012年期间，对南非七个省份商业家禽气管拭子样本中的36份鸡毒支原体DNA提取物进行了分子分析。通过聚合酶链反应以及对16S - 23S rRNA基因间隔区（IGSR）、鸡毒支原体细胞黏附素2（mgc2）、MGA_0319和gapA基因区域进行序列分析，鉴定出了12种独特的鸡毒支原体基因型。这些基因型的DNA序列与一个由来自其他国家的序列、疫苗和参考菌株组成的数据库中的鸡毒支原体分离株序列不同。最常见的基因型（SA - WT#7）在五个省份的商业肉鸡、肉种鸡和蛋鸡样本中均有检测到。南非鸡毒支原体序列与南非市售活疫苗的序列更为相似，但与未在南非注册使用的F株疫苗序列不同。三种南非基因型的IGSR、mgc2或MGA_0319序列与ts - 11疫苗株相同，因此需要结合mgc2和IGSR靶向测序来区分南非野生型基因型与ts - 11疫苗。为了鉴定和区分所有12种野生型，需要对mgc2、IGSR和MGA_0319进行测序。gapA测序在菌株区分方面效果最差。本研究为鸡毒支原体序列数据库的开发提供了一个模型，并说明了其在表征鸡毒支原体基因型、选择诊断测试以及更好地了解鸡毒支原体流行病学方面的应用。
