Department of Chemistry, University at Albany, 1400 Washington Avenue, Albany, NY 12222, USA.
Forensic Sci Int. 2013 Sep 10;231(1-3):157-66. doi: 10.1016/j.forsciint.2013.04.033. Epub 2013 May 25.
Raman spectroscopy has demonstrated remarkable capabilities in identifying blood in controlled laboratory conditions. However, substrate interference presents a significant challenge toward characterizing body fluid traces with Raman spectroscopy at a crime scene. Here, several possible solutions are explored, including the selection of laser excitation, isolating the signal of blood using spectral subtraction and using a favorable substrate for collection which minimizes interference. Simulated blood stain evidence was prepared and analyzed using a Raman microscope with variable laser capabilities. It is shown that the best approach for detecting blood depends on the nature of the substrate and the type of interference encountered.
拉曼光谱在识别受控实验室条件下的血液方面表现出了显著的能力。然而，在犯罪现场用拉曼光谱对体液痕迹进行特征分析时，基质干扰是一个重大的挑战。这里，我们探索了几种可能的解决方案，包括选择激光激发、使用光谱减除法分离血液信号以及使用对收集干扰最小的有利基质。使用具有可变激光功能的拉曼显微镜对模拟血斑证据进行了制备和分析。结果表明，检测血液的最佳方法取决于基质的性质和遇到的干扰类型。
